DC Field | Value | Language |
---|---|---|
dc.contributor.author | Wang, Fei | ko |
dc.contributor.author | Wang, Lianrong | ko |
dc.contributor.author | Zou, Xuan | ko |
dc.contributor.author | Duan, Suling | ko |
dc.contributor.author | Li, Zhiqiang | ko |
dc.contributor.author | Deng, Zixin | ko |
dc.contributor.author | Luo, Jie | ko |
dc.contributor.author | Lee, Sang Yup | ko |
dc.contributor.author | Chen, Shi | ko |
dc.date.accessioned | 2019-08-22T02:20:04Z | - |
dc.date.available | 2019-08-22T02:20:04Z | - |
dc.date.created | 2019-08-19 | - |
dc.date.created | 2019-08-19 | - |
dc.date.issued | 2019-09 | - |
dc.identifier.citation | BIOTECHNOLOGY ADVANCES, v.37, no.5, pp.708 - 729 | - |
dc.identifier.issn | 0734-9750 | - |
dc.identifier.uri | http://hdl.handle.net/10203/264383 | - |
dc.description.abstract | Clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein (Cas) systems, especially type II (Cas9) systems, have been widely used in gene/genome targeting. Modifications of Cas9 enable these systems to become platforms for precise DNA manipulations. However, the utilization of CRISPR-Cas systems in RNA targeting remains preliminary. The discovery of type VI CRISPR-Cas systems (Cas13) shed light on RNA-guided RNA targeting. Cas13d, the smallest Cas13 protein, with a length of only similar to 930 amino acids, is a promising platform for RNA targeting compatible with viral delivery systems. Much effort has also been made to develop Cas9, Cas13a and Cas13b applications for RNA-guided RNA targeting. The discovery of new RNA targeting CRISPR-Cas systems as well as the development of RNA-targeting platforms with Cas9 and Cas13 will promote RNA-targeting technology substantially. Here, we review new advances in RNA-targeting CRISPR-Cas systems as well as advances in applications of these systems in RNA targeting, tracking and editing. We also compare these Cas protein-based technologies with traditional technologies for RNA targeting, tracking and editing. Finally, we discuss remaining questions and prospects for the future. | - |
dc.language | English | - |
dc.publisher | PERGAMON-ELSEVIER SCIENCE LTD | - |
dc.title | Advances in CRISPR-Cas systems for RNA targeting, tracking and editing | - |
dc.type | Article | - |
dc.identifier.wosid | 000477917000007 | - |
dc.identifier.scopusid | 2-s2.0-85063660374 | - |
dc.type.rims | ART | - |
dc.citation.volume | 37 | - |
dc.citation.issue | 5 | - |
dc.citation.beginningpage | 708 | - |
dc.citation.endingpage | 729 | - |
dc.citation.publicationname | BIOTECHNOLOGY ADVANCES | - |
dc.identifier.doi | 10.1016/j.biotechadv.2019.03.016 | - |
dc.contributor.localauthor | Lee, Sang Yup | - |
dc.contributor.nonIdAuthor | Wang, Fei | - |
dc.contributor.nonIdAuthor | Wang, Lianrong | - |
dc.contributor.nonIdAuthor | Zou, Xuan | - |
dc.contributor.nonIdAuthor | Duan, Suling | - |
dc.contributor.nonIdAuthor | Li, Zhiqiang | - |
dc.contributor.nonIdAuthor | Deng, Zixin | - |
dc.contributor.nonIdAuthor | Luo, Jie | - |
dc.contributor.nonIdAuthor | Chen, Shi | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Review | - |
dc.subject.keywordAuthor | CRISPR-Cas systems | - |
dc.subject.keywordAuthor | RNA targeting | - |
dc.subject.keywordAuthor | RNA tracking | - |
dc.subject.keywordAuthor | RNA editing | - |
dc.subject.keywordPlus | DOUBLE-STRANDED-RNA | - |
dc.subject.keywordPlus | LINKED MOLECULAR BEACONS | - |
dc.subject.keywordPlus | NUCLEIC-ACID DETECTION | - |
dc.subject.keywordPlus | MESSENGER-RNA | - |
dc.subject.keywordPlus | GENE-EXPRESSION | - |
dc.subject.keywordPlus | CRYSTAL-STRUCTURE | - |
dc.subject.keywordPlus | GUIDED ENDONUCLEASE | - |
dc.subject.keywordPlus | SPACER ACQUISITION | - |
dc.subject.keywordPlus | DNA CLEAVAGE | - |
dc.subject.keywordPlus | CMR COMPLEX | - |
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