DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Hye Mi | ko |
dc.contributor.author | Chae, Tong Un | ko |
dc.contributor.author | Choi, So Young | ko |
dc.contributor.author | Kim, Won Jun | ko |
dc.contributor.author | Lee, Sang Yup | ko |
dc.date.accessioned | 2019-07-18T05:31:19Z | - |
dc.date.available | 2019-07-18T05:31:19Z | - |
dc.date.created | 2019-07-15 | - |
dc.date.created | 2019-07-15 | - |
dc.date.issued | 2019-07 | - |
dc.identifier.citation | NATURE CHEMICAL BIOLOGY, v.15, no.7, pp.721 - 729 | - |
dc.identifier.issn | 1552-4450 | - |
dc.identifier.uri | http://hdl.handle.net/10203/263296 | - |
dc.description.abstract | Production of free fatty acids (FFAs) and derivatives from renewable non-food biomass by microbial fermentation is of great interest. Here, we report the development of engineered Rhodococcus opacus strains producing FFAs, fatty acid ethyl esters (FAEEs) and long-chain hydrocarbons (LCHCs). Culture conditions were optimized to produce 82.9 g l(-1) of triacylglycerols from glucose, and an engineered strain with acyl-coenzyme A (CoA) synthetases deleted, overexpressing three lipases with lipase-specific foldase produced 50.2 g l(-1) of FFAs. Another engineered strain with acyl-CoA dehydrogenases deleted, overexpressing lipases, foldase, acyl-CoA synthetase and heterologous aldehyde/alcohol dehydrogenase and wax ester synthase produced 21.3 g l(-1) of FAEEs. A third engineered strain with acyl-CoA dehydrogenases and alkane-1 monooxygenase deleted, overexpressing lipases, foldase, acyl-CoA synthetase and heterologous acyl-CoA reductase, acyl-ACP reductase and aldehyde deformylating oxygenase produced 5.2g l(-1) of LCHCs. Metabolic engineering strategies and engineered strains developed here may help establish oleaginous biorefinery platforms for the sustainable production of chemicals and fuels. | - |
dc.language | English | - |
dc.publisher | NATURE PUBLISHING GROUP | - |
dc.title | Engineering of an oleaginous bacterium for the production of fatty acids and fuels | - |
dc.type | Article | - |
dc.identifier.wosid | 000472625600016 | - |
dc.identifier.scopusid | 2-s2.0-85067878142 | - |
dc.type.rims | ART | - |
dc.citation.volume | 15 | - |
dc.citation.issue | 7 | - |
dc.citation.beginningpage | 721 | - |
dc.citation.endingpage | 729 | - |
dc.citation.publicationname | NATURE CHEMICAL BIOLOGY | - |
dc.identifier.doi | 10.1038/s41589-019-0295-5 | - |
dc.contributor.localauthor | Lee, Sang Yup | - |
dc.contributor.nonIdAuthor | Choi, So Young | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | RHODOCOCCUS-OPACUS PD630 | - |
dc.subject.keywordPlus | MICROBIAL-PRODUCTION | - |
dc.subject.keywordPlus | EUTROPHA H16 | - |
dc.subject.keywordPlus | BIOSYNTHESIS | - |
dc.subject.keywordPlus | BIOFUELS | - |
dc.subject.keywordPlus | GENE | - |
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