DC Field | Value | Language |
---|---|---|
dc.contributor.author | Han, Jeongran | ko |
dc.contributor.author | Lee, Ji Youn | ko |
dc.contributor.author | Bae, Young-Kyung | ko |
dc.date.accessioned | 2019-07-05T07:10:13Z | - |
dc.date.available | 2019-07-05T07:10:13Z | - |
dc.date.created | 2019-07-01 | - |
dc.date.created | 2019-07-01 | - |
dc.date.issued | 2019-08 | - |
dc.identifier.citation | BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS, v.1863, no.8, pp.1235 - 1242 | - |
dc.identifier.issn | 0304-4165 | - |
dc.identifier.uri | http://hdl.handle.net/10203/263008 | - |
dc.description.abstract | Background: Regulated cell death plays an essential role in various biological processes, leading to the development of a number of methods to detect and quantitatively measure cells exhibiting decreased viability due to either apoptosis or necrosis. Methods and results: When cytotoxicity is induced by anti-cancer chemicals, human cell lines exhibit specific features, including dampened cell proliferation and lost plasma membrane asymmetry, presenting distinct sensitivity. In this study, we report a set of novel digital PCR (dPCR) assays to quantitatively measure the degree of cell death. These dPCR assays are designed to quantify targets of increasing sizes within the RNase P (RP) gene locus. The ratio between short and long target copy numbers implies the degree of DNA fragmentation, which we name the RP fragmentation index. Conclusions: Compared to other conventional quantitative methods, the RP fragmentation index using cellular DNA represents a valid indicator in the measurement of the degree of cell death. General significance: The demonstrated dPCR assays can precisely assess DNA fragmentation that quantitatively reflects the degree of cytotoxicity. | - |
dc.language | English | - |
dc.publisher | ELSEVIER SCIENCE BV | - |
dc.title | Application of digital PCR for assessing DNA fragmentation in cytotoxicity response | - |
dc.type | Article | - |
dc.identifier.wosid | 000471356900001 | - |
dc.identifier.scopusid | 2-s2.0-85065259201 | - |
dc.type.rims | ART | - |
dc.citation.volume | 1863 | - |
dc.citation.issue | 8 | - |
dc.citation.beginningpage | 1235 | - |
dc.citation.endingpage | 1242 | - |
dc.citation.publicationname | BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS | - |
dc.identifier.doi | 10.1016/j.bbagen.2019.05.001 | - |
dc.contributor.localauthor | Han, Jeongran | - |
dc.contributor.nonIdAuthor | Lee, Ji Youn | - |
dc.contributor.nonIdAuthor | Bae, Young-Kyung | - |
dc.description.isOpenAccess | Y | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | DNA fragmentation | - |
dc.subject.keywordAuthor | Digital PCR | - |
dc.subject.keywordAuthor | Cytotoxicity | - |
dc.subject.keywordAuthor | Regulated cell death | - |
dc.subject.keywordPlus | CELL-FREE DNA | - |
dc.subject.keywordPlus | FREE CIRCULATING DNA | - |
dc.subject.keywordPlus | HUMAN BLOOD-PLASMA | - |
dc.subject.keywordPlus | ABSOLUTE QUANTIFICATION | - |
dc.subject.keywordPlus | INCREASED INTEGRITY | - |
dc.subject.keywordPlus | CANCER | - |
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