DC Field | Value | Language |
---|---|---|
dc.contributor.author | Xiong, Kai | ko |
dc.contributor.author | Marquart, Kim Fabiano | ko |
dc.contributor.author | Karottki, Karen Julie la Cour | ko |
dc.contributor.author | Li, Shangzhong | ko |
dc.contributor.author | Shamie, Isaac | ko |
dc.contributor.author | Lee, Jae Seong | ko |
dc.contributor.author | Gerling, Signe | ko |
dc.contributor.author | Yeo, Nan Cher | ko |
dc.contributor.author | Chavez, Alejandro | ko |
dc.contributor.author | Lee, Gyun Min | ko |
dc.contributor.author | Lewis, Nathan E. | ko |
dc.contributor.author | Kildegaard, Helene Faustrup | ko |
dc.date.accessioned | 2019-06-24T01:30:04Z | - |
dc.date.available | 2019-06-24T01:30:04Z | - |
dc.date.created | 2019-06-10 | - |
dc.date.issued | 2019-06 | - |
dc.identifier.citation | BIOTECHNOLOGY AND BIOENGINEERING, v.116, no.7, pp.1813 - 1819 | - |
dc.identifier.issn | 0006-3592 | - |
dc.identifier.uri | http://hdl.handle.net/10203/262788 | - |
dc.description.abstract | Chinese hamster ovary (CHO) cells are widely used for biopharmaceutical protein production. One challenge limiting CHO cell productivity is apoptosis stemming from cellular stress during protein production. Here we applied CRISPR interference (CRISPRi) to downregulate the endogenous expression of apoptotic genes Bak, Bax, and Casp3 in CHO cells. In addition to reduced apoptosis, mitochondrial membrane integrity was improved and the caspase activity was reduced. Moreover, we optimized the CRISPRi system to enhance the gene repression efficiency in CHO cells by testing different repressor fusion types. An improved Cas9 repressor has been identified by applying C-terminal fusion of a bipartite repressor domain, KRAB-MeCP2, to nuclease-deficient Cas9. These results collectively demonstrate that CHO cells can be rescued from cell apoptosis by targeted gene repression using the CRISPRi system. | - |
dc.language | English | - |
dc.publisher | WILEY | - |
dc.title | Reduced apoptosis in Chinese hamster ovary cells via optimized CRISPR interference | - |
dc.type | Article | - |
dc.identifier.wosid | 000469929000021 | - |
dc.identifier.scopusid | 2-s2.0-85066475656 | - |
dc.type.rims | ART | - |
dc.citation.volume | 116 | - |
dc.citation.issue | 7 | - |
dc.citation.beginningpage | 1813 | - |
dc.citation.endingpage | 1819 | - |
dc.citation.publicationname | BIOTECHNOLOGY AND BIOENGINEERING | - |
dc.identifier.doi | 10.1002/bit.26969 | - |
dc.contributor.localauthor | Lee, Gyun Min | - |
dc.contributor.nonIdAuthor | Xiong, Kai | - |
dc.contributor.nonIdAuthor | Marquart, Kim Fabiano | - |
dc.contributor.nonIdAuthor | Karottki, Karen Julie la Cour | - |
dc.contributor.nonIdAuthor | Li, Shangzhong | - |
dc.contributor.nonIdAuthor | Shamie, Isaac | - |
dc.contributor.nonIdAuthor | Lee, Jae Seong | - |
dc.contributor.nonIdAuthor | Gerling, Signe | - |
dc.contributor.nonIdAuthor | Yeo, Nan Cher | - |
dc.contributor.nonIdAuthor | Chavez, Alejandro | - |
dc.contributor.nonIdAuthor | Lewis, Nathan E. | - |
dc.contributor.nonIdAuthor | Kildegaard, Helene Faustrup | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | apoptosis | - |
dc.subject.keywordAuthor | Bak | - |
dc.subject.keywordAuthor | Bax | - |
dc.subject.keywordAuthor | Casp3 | - |
dc.subject.keywordAuthor | caspase | - |
dc.subject.keywordAuthor | CHO | - |
dc.subject.keywordAuthor | CRISPRi | - |
dc.subject.keywordAuthor | mitochondrial membrane integrity | - |
dc.subject.keywordPlus | BATCH CULTURES | - |
dc.subject.keywordPlus | RNA | - |
dc.subject.keywordPlus | BAX | - |
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