DC Field | Value | Language |
---|---|---|
dc.contributor.author | Sung, Daekyung | ko |
dc.contributor.author | Yang, Sung | ko |
dc.contributor.author | Park, Jeong Won | ko |
dc.contributor.author | Jon, Sangyong | ko |
dc.date.accessioned | 2019-04-15T15:12:03Z | - |
dc.date.available | 2019-04-15T15:12:03Z | - |
dc.date.created | 2013-02-05 | - |
dc.date.issued | 2013-08 | - |
dc.identifier.citation | BIOMEDICAL MICRODEVICES, v.15, no.4, pp.691 - 698 | - |
dc.identifier.issn | 1387-2176 | - |
dc.identifier.uri | http://hdl.handle.net/10203/254763 | - |
dc.description.abstract | Our research efforts have been devoted to development of nanobead multilayer-based sensitive immunoassays on cyclic olefin copolymer (COC) plastic surfaces. To facilitate nanobead attachment and impart antibiofouling properties to a COC substrate, we used an amphiphilic copolymer comprising benzyl, polyethylene glycol, and reactive ester moieties to coat the hydrophobic COC surface in an aqueous environment. Subsequently, NH2-modified polystyrene nanobeads were reacted with the polymer-coated COC surface and further assembled into multilayers that increased the overall surface area available for attaching capture antibodies. After treatment of the nanobead multilayers with an amine-reactive homobifunctional crosslinker, a model capture antibody (anti-rabbit IgG) was covalently immobilized onto the activated surface of nanobeads. Finally, a sandwich immunoassay was carried out using rabbit IgG as a target analyte and rhodamine-labeled anti-rabbit IgG as a probe. Compared with a nanobead-free, polymer-coated COC surface, the nanobead multilayer-based immunoassay exhibited similar to 4-fold higher fluorescence intensity. In addition, our nanobead-based assay system exhibited a wide dynamic range of detection (0.1 to 1,000 ng/mL) and high specificity for rabbit IgG. Furthermore, much better detection sensitivity for rabbit IgG was attained in the nanobead multilayer-based immunoassay than with a conventional ELISA system (0.1 ng/mL versus 10 ng/mL), indicating the potential value of the proposed immunoassay system in plastic-based portable biochip applications. | - |
dc.language | English | - |
dc.publisher | SPRINGER | - |
dc.subject | FACILE IMMOBILIZATION | - |
dc.subject | PROTEIN MICROARRAYS | - |
dc.subject | SOLID SUPPORTS | - |
dc.subject | WHOLE-BLOOD | - |
dc.subject | PERFORMANCE | - |
dc.subject | STRATEGIES | - |
dc.subject | BIOCHIPS | - |
dc.subject | POLYMERS | - |
dc.subject | SAMPLES | - |
dc.title | High-density immobilization of antibodies onto nanobead-coated cyclic olefin copolymer plastic surfaces for application as a sensitive immunoassay chip | - |
dc.type | Article | - |
dc.identifier.wosid | 000323909200013 | - |
dc.identifier.scopusid | 2-s2.0-84880729605 | - |
dc.type.rims | ART | - |
dc.citation.volume | 15 | - |
dc.citation.issue | 4 | - |
dc.citation.beginningpage | 691 | - |
dc.citation.endingpage | 698 | - |
dc.citation.publicationname | BIOMEDICAL MICRODEVICES | - |
dc.identifier.doi | 10.1007/s10544-012-9732-x | - |
dc.contributor.localauthor | Jon, Sangyong | - |
dc.contributor.nonIdAuthor | Sung, Daekyung | - |
dc.contributor.nonIdAuthor | Yang, Sung | - |
dc.contributor.nonIdAuthor | Park, Jeong Won | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | Cyclic olefin copolymer | - |
dc.subject.keywordAuthor | Surface modification | - |
dc.subject.keywordAuthor | Antibiofouling polymers | - |
dc.subject.keywordAuthor | Polystyrene nanobeads | - |
dc.subject.keywordAuthor | Antibody immobilization | - |
dc.subject.keywordAuthor | Immunoassay | - |
dc.subject.keywordPlus | FACILE IMMOBILIZATION | - |
dc.subject.keywordPlus | PROTEIN MICROARRAYS | - |
dc.subject.keywordPlus | SOLID SUPPORTS | - |
dc.subject.keywordPlus | WHOLE-BLOOD | - |
dc.subject.keywordPlus | PERFORMANCE | - |
dc.subject.keywordPlus | STRATEGIES | - |
dc.subject.keywordPlus | BIOCHIPS | - |
dc.subject.keywordPlus | POLYMERS | - |
dc.subject.keywordPlus | SAMPLES | - |
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