DC Field | Value | Language |
---|---|---|
dc.contributor.author | Choi, Hyeokjune | ko |
dc.contributor.author | Lee, Jeong Min | ko |
dc.contributor.author | Jung, Yongwon | ko |
dc.date.accessioned | 2019-04-15T14:15:08Z | - |
dc.date.available | 2019-04-15T14:15:08Z | - |
dc.date.created | 2019-04-03 | - |
dc.date.created | 2019-04-03 | - |
dc.date.issued | 2019-03 | - |
dc.identifier.citation | ADVANCED BIOSYSTEMS, v.3, no.3 | - |
dc.identifier.issn | 2366-7478 | - |
dc.identifier.uri | http://hdl.handle.net/10203/253988 | - |
dc.description.abstract | With high brightness and photostability, quantum dots (QDs) are potent probes for long-term imaging of dynamic cell surface proteins, but practical methods to covalently label QDs to target proteins for stable imaging are largely lacking. Here, a small covalent-bond forming protein (Covalent-avidin)/peptide pair is introduced, which provides a recombinant protein-based rapid and covalent QD labeling strategy. Covalent-avidin is constructed by optimized fusion of circular permuted monomeric avidin to SpyCatcher, which forms an isopeptide bond with the SpyTag peptide. Covalent-avidin-conjugated QDs allow for strong and irreversible QD labeling to the biotinylated SpyTag-fused adrenergic receptor on live cells in 2 min. In addition, QDs with only a minimum number of conjugated Covalent-avidin show more stable receptor labeling than commercially available streptavidin-conjugated QDs, also with minimal unwanted clustering of labeled receptors. Monomeric Covalent-avidin will be a valuable protein linker for diverse other nanolabeling structures with beneficial properties such as covalent linkages and facile valency control. | - |
dc.language | English | - |
dc.publisher | WILEY-V C H VERLAG GMBH | - |
dc.title | Monomeric Covalent-Avidin for Rapid and Covalent Labeling of Quantum Dots to Cell Surface Proteins | - |
dc.type | Article | - |
dc.identifier.wosid | 000461872900001 | - |
dc.identifier.scopusid | 2-s2.0-85063108713 | - |
dc.type.rims | ART | - |
dc.citation.volume | 3 | - |
dc.citation.issue | 3 | - |
dc.citation.publicationname | ADVANCED BIOSYSTEMS | - |
dc.identifier.doi | 10.1002/adbi.201800288 | - |
dc.contributor.localauthor | Jung, Yongwon | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | avidin | - |
dc.subject.keywordAuthor | covalent label | - |
dc.subject.keywordAuthor | membrane proteins | - |
dc.subject.keywordAuthor | protein engineering | - |
dc.subject.keywordAuthor | quantum dots | - |
dc.subject.keywordPlus | MONOVALENT | - |
dc.subject.keywordPlus | RECEPTORS | - |
dc.subject.keywordPlus | COMPACT | - |
dc.subject.keywordPlus | LIGASE | - |
dc.subject.keywordPlus | SIZE | - |
dc.subject.keywordPlus | TAG | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.