Nx-2,2,4,6,7-Pentamethyldihydrobenzofuran-5-sulfonyl (Nx-Pbf)-protected oligoarginine was directly
conjugated to poly(amino acid) derivatives modified with a long alkyl chain. The final concentration of
conjugated peptides was easily controlled by the feed ratio of oligoarginine to polymer backbone and a final
soluble polymeric system was obtained by the deprotection of Nx-Pbf groups. The polymeric conjugates
formed stable self-aggregates of size range of 8–40 nm in aqueous solution and effectively internalized into
HeLa cells by adsorptive endocytosis.