DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Soo Jin | ko |
dc.contributor.author | Kim, Ho Min | ko |
dc.date.accessioned | 2019-03-08T15:37:24Z | - |
dc.date.available | 2019-03-08T15:37:24Z | - |
dc.date.created | 2017-03-03 | - |
dc.date.issued | 2017-01 | - |
dc.identifier.citation | BMB REPORTS, v.50, no.2, pp.55 - 57 | - |
dc.identifier.issn | 1976-6696 | - |
dc.identifier.uri | http://hdl.handle.net/10203/251124 | - |
dc.description.abstract | Toll-like receptor 4 (TLR4) together with MD2, one of the key pattern recognition receptors for a pathogen-associated molecular pattern, activates innate immunity by recognizing lipopolysaccharide (LPS) of Gram-negative bacteria. Although LBP and CD14 catalyze LPS transfer to the TLR4/MD2 complex, the detail mechanisms underlying this dynamic LPS transfer remain elusive. Using negative-stain electron microscopy, we visualized the dynamic intermediate complexes during LPS transfer-LBP/LPS micelles and ternary CD14/LBP/ LPS micelle complexes. We also reconstituted the entire cascade of LPS transfer to TLR4/MD2 in a total internal reflection fluorescence (TIRF) microscope for a single molecule fluorescence analysis. These analyses reveal longitudinal LBP binding to the surface of LPS micelles and multi-round binding/unbinding of CD14 to single LBP/LPS micelles via key charged residues on LBP and CD14. Finally, we reveal that a single LPS molecule bound to CD14 is transferred to TLR4/MD2 in a TLR4-dependent manner. These discoveries, which clarify the molecular mechanism of dynamic LPS transfer to TLR4/MD2 via LBP and CD14, provide novel insights into the initiation of innate immune responses. | - |
dc.language | English | - |
dc.publisher | KOREAN SOCIETY BIOCHEMISTRY & MOLECULAR BIOLOGY | - |
dc.title | Dynamic lipopolysaccharide transfer cascade to TLR4/MD2 complex via LBP and CD14 | - |
dc.type | Article | - |
dc.identifier.wosid | 000396828300001 | - |
dc.identifier.scopusid | 2-s2.0-85014092280 | - |
dc.type.rims | ART | - |
dc.citation.volume | 50 | - |
dc.citation.issue | 2 | - |
dc.citation.beginningpage | 55 | - |
dc.citation.endingpage | 57 | - |
dc.citation.publicationname | BMB REPORTS | - |
dc.identifier.doi | 10.5483/BMBRep.2017.50.2.011.007 | - |
dc.contributor.localauthor | Kim, Ho Min | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | CD14 | - |
dc.subject.keywordAuthor | Innate immunity | - |
dc.subject.keywordAuthor | LBP | - |
dc.subject.keywordAuthor | LPS transfer | - |
dc.subject.keywordAuthor | Single molecule fluorescence analysis | - |
dc.subject.keywordAuthor | TLR4/MD2 | - |
dc.subject.keywordAuthor | Transmission electron microscopy | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.