DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, SH | ko |
dc.contributor.author | Noh, J | ko |
dc.contributor.author | Jeon, MK | ko |
dc.contributor.author | Kim, KW | ko |
dc.contributor.author | Lee, LP | ko |
dc.contributor.author | Woo, Seong-Ihl | ko |
dc.date.accessioned | 2019-03-06T09:37:38Z | - |
dc.date.available | 2019-03-06T09:37:38Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2006-03 | - |
dc.identifier.citation | JOURNAL OF MICROMECHANICS AND MICROENGINEERING, v.16, no.3, pp.526 - 530 | - |
dc.identifier.issn | 0960-1317 | - |
dc.identifier.uri | http://hdl.handle.net/10203/250603 | - |
dc.description.abstract | The temperature distribution inside the microchannel was first measured using a micro-Raman spectroscopy system. Polymerase chain reaction (PCR) inside the microchannel, which is very sensitive to temperature change, was chosen to validate a novel thermometry. The micro PCR chip was fabricated by silicon micromachining. The microchip consisted of a microchannel inside the chip, a Pt film heater and a Pt film sensor on the silicon surface. The O-H stretch vibration mode of water was analyzed using a micro-Raman spectroscopy system. Its spectral changes were calibrated with temperature. The intensity ratio of the hydrogen-bonded (HB) to non-hydrogen-bonded (NHB) O-H stretch vibration modes has a linear relation with temperature. The temperature of the microchannel controlled at 55 degrees C varied from 53.6 to 54.8 degrees C depending on the location of the microchannel. | - |
dc.language | English | - |
dc.publisher | IOP PUBLISHING LTD | - |
dc.subject | CONTINUOUS-FLOW PCR | - |
dc.subject | ELECTROPHORESIS CAPILLARIES | - |
dc.subject | AMPLIFICATION | - |
dc.subject | WATER | - |
dc.title | Micro-Raman thermometry for measuring the temperature distribution inside the microchannel of a polymerase chain reaction chip | - |
dc.type | Article | - |
dc.identifier.wosid | 000237073000007 | - |
dc.identifier.scopusid | 2-s2.0-33344456867 | - |
dc.type.rims | ART | - |
dc.citation.volume | 16 | - |
dc.citation.issue | 3 | - |
dc.citation.beginningpage | 526 | - |
dc.citation.endingpage | 530 | - |
dc.citation.publicationname | JOURNAL OF MICROMECHANICS AND MICROENGINEERING | - |
dc.identifier.doi | 10.1088/0960-1317/16/3/007 | - |
dc.contributor.localauthor | Woo, Seong-Ihl | - |
dc.contributor.nonIdAuthor | Kim, SH | - |
dc.contributor.nonIdAuthor | Noh, J | - |
dc.contributor.nonIdAuthor | Jeon, MK | - |
dc.contributor.nonIdAuthor | Kim, KW | - |
dc.contributor.nonIdAuthor | Lee, LP | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | CONTINUOUS-FLOW PCR | - |
dc.subject.keywordPlus | ELECTROPHORESIS CAPILLARIES | - |
dc.subject.keywordPlus | AMPLIFICATION | - |
dc.subject.keywordPlus | WATER | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.