DC Field | Value | Language |
---|---|---|
dc.contributor.author | Cho, Chang Hyun | ko |
dc.contributor.author | Kwon, Seyong | ko |
dc.contributor.author | Kim, Segi | ko |
dc.contributor.author | Hong, Yoonmi | ko |
dc.contributor.author | Kim, Pilnam | ko |
dc.contributor.author | Lee, Eun Sook | ko |
dc.contributor.author | Park, Je-Kyun | ko |
dc.date.accessioned | 2018-10-19T00:32:44Z | - |
dc.date.available | 2018-10-19T00:32:44Z | - |
dc.date.created | 2018-08-06 | - |
dc.date.created | 2018-08-06 | - |
dc.date.created | 2018-08-06 | - |
dc.date.issued | 2018-07 | - |
dc.identifier.citation | BIOMICROFLUIDICS, v.12, no.4, pp.044110 | - |
dc.identifier.issn | 1932-1058 | - |
dc.identifier.uri | http://hdl.handle.net/10203/245937 | - |
dc.description.abstract | We present here a novel microfluidic platform that can perform microfluidic on-chip immunohistochemistry (IHC) processes on a formalin-fixed paraffin-embedded section slide. Unlike previous microfluidic IHC studies, our microfluidic chip made of organic solvent-resistant polyurethane acrylate (PUA) is capable of conducting on-chip IHC processes consecutively. A narrow channel wall structure of the PUA chip shows effective sealing by pressure-based reversible assembly with a section slide. We performed both on-chip IHC and conventional IHC processes and compared the IHC results based on the immunostaining intensity. The result showed that the effects of the on-chip deparaffinization, antigen retrieval, and immunoreaction processes on the IHC result were equivalent to conventional methods while reducing the total process time to less than 1/2. The experiment with breast cancer tissue shows that human epidermal growth factor receptor 2 (HER2) classification can be performed by obtaining a clearly distinguishable immunostaining intensity according to the HER2 expression level. We expect our on-chip microfluidic platform to provide a facile technique suitable for miniaturized, automated, and precise diagnostic devices, including a point-of-care device. Published by AIP Publishing. | - |
dc.language | English | - |
dc.publisher | AMER INST PHYSICS | - |
dc.title | Microfluidic on-chip immunohistochemistry directly from a paraffin-embedded section | - |
dc.type | Article | - |
dc.identifier.wosid | 000443760100030 | - |
dc.identifier.scopusid | 2-s2.0-85050492966 | - |
dc.type.rims | ART | - |
dc.citation.volume | 12 | - |
dc.citation.issue | 4 | - |
dc.citation.beginningpage | 044110 | - |
dc.citation.publicationname | BIOMICROFLUIDICS | - |
dc.identifier.doi | 10.1063/1.5042347 | - |
dc.contributor.localauthor | Kim, Pilnam | - |
dc.contributor.localauthor | Park, Je-Kyun | - |
dc.contributor.nonIdAuthor | Lee, Eun Sook | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article; Proceedings Paper | - |
dc.subject.keywordPlus | BREAST-CANCER | - |
dc.subject.keywordPlus | ANTIGEN RETRIEVAL | - |
dc.subject.keywordPlus | BIOMARKERS | - |
dc.subject.keywordPlus | RECEPTOR | - |
dc.subject.keywordPlus | IMMUNOCYTOCHEMISTRY | - |
dc.subject.keywordPlus | CARCINOMAS | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | PLATFORM | - |
dc.subject.keywordPlus | CELLS | - |
dc.subject.keywordPlus | TOOL | - |
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