Hybrid Microscopy: Enabling Inexpensive High-Performance Imaging through Combined Physical and Optical Magnifications

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dc.contributor.authorZhang, Yu Shrikeko
dc.contributor.authorChang, Jae-Byumko
dc.contributor.authorAlvarez, Mario Moisesko
dc.contributor.authorTrujillo-de Santiago, Grisselko
dc.contributor.authorAleman, Julioko
dc.contributor.authorBatzaya, Byambaako
dc.contributor.authorKrishnadoss, Vaishaliko
dc.contributor.authorRamanujam, Aishwarya Aravamudhanko
dc.contributor.authorKazemzadeh-Narbat, Mehdiko
dc.contributor.authorChen, Feiko
dc.contributor.authorTillberg, Paul W.ko
dc.contributor.authorDokmeci, Mehmet Remziko
dc.contributor.authorBoyden, Edward S.ko
dc.contributor.authorKhademhosseini, Aliko
dc.date.accessioned2018-09-18T06:24:53Z-
dc.date.available2018-09-18T06:24:53Z-
dc.date.created2018-09-04-
dc.date.created2018-09-04-
dc.date.created2018-09-04-
dc.date.issued2016-03-
dc.identifier.citationSCIENTIFIC REPORTS, v.6-
dc.identifier.issn2045-2322-
dc.identifier.urihttp://hdl.handle.net/10203/245591-
dc.description.abstractTo date, much effort has been expended on making high-performance microscopes through better instrumentation. Recently, it was discovered that physical magnification of specimens was possible, through a technique called expansion microscopy (ExM), raising the question of whether physical magnification, coupled to inexpensive optics, could together match the performance of high-end optical equipment, at a tiny fraction of the price. Here we show that such "hybrid microscopy" methods-combining physical and optical magnifications-can indeed achieve high performance at low cost. By physically magnifying objects, then imaging them on cheap miniature fluorescence microscopes ("mini-microscopes"), it is possible to image at a resolution comparable to that previously attainable only with benchtop microscopes that present costs orders of magnitude higher. We believe that this unprecedented hybrid technology that combines expansion microscopy, based on physical magnification, and mini-microscopy, relying on conventional optics-a process we refer to as Expansion Mini-Microscopy (ExMM)-is a highly promising alternative method for performing cost-effective, high-resolution imaging of biological samples. With further advancement of the technology, we believe that ExMM will find widespread applications for high-resolution imaging particularly in research and healthcare scenarios in undeveloped countries or remote places.-
dc.languageEnglish-
dc.publisherNATURE PUBLISHING GROUP-
dc.subjectRECONSTRUCTION MICROSCOPY-
dc.subjectSTIMULATED-EMISSION-
dc.subjectANTIBODY FRAGMENT-
dc.subjectMINI-MICROSCOPE-
dc.subjectON-CHIP-
dc.subjectCELLS-
dc.subjectPROBES-
dc.subjectSTORM-
dc.subjectLIMIT-
dc.titleHybrid Microscopy: Enabling Inexpensive High-Performance Imaging through Combined Physical and Optical Magnifications-
dc.typeArticle-
dc.identifier.wosid000372028800001-
dc.identifier.scopusid2-s2.0-84960968910-
dc.type.rimsART-
dc.citation.volume6-
dc.citation.publicationnameSCIENTIFIC REPORTS-
dc.identifier.doi10.1038/srep22691-
dc.contributor.localauthorChang, Jae-Byum-
dc.contributor.nonIdAuthorZhang, Yu Shrike-
dc.contributor.nonIdAuthorAlvarez, Mario Moises-
dc.contributor.nonIdAuthorTrujillo-de Santiago, Grissel-
dc.contributor.nonIdAuthorAleman, Julio-
dc.contributor.nonIdAuthorBatzaya, Byambaa-
dc.contributor.nonIdAuthorKrishnadoss, Vaishali-
dc.contributor.nonIdAuthorRamanujam, Aishwarya Aravamudhan-
dc.contributor.nonIdAuthorKazemzadeh-Narbat, Mehdi-
dc.contributor.nonIdAuthorChen, Fei-
dc.contributor.nonIdAuthorTillberg, Paul W.-
dc.contributor.nonIdAuthorDokmeci, Mehmet Remzi-
dc.contributor.nonIdAuthorBoyden, Edward S.-
dc.contributor.nonIdAuthorKhademhosseini, Ali-
dc.description.isOpenAccessN-
dc.type.journalArticleArticle-
dc.subject.keywordPlusRECONSTRUCTION MICROSCOPY-
dc.subject.keywordPlusSTIMULATED-EMISSION-
dc.subject.keywordPlusANTIBODY FRAGMENT-
dc.subject.keywordPlusMINI-MICROSCOPE-
dc.subject.keywordPlusON-CHIP-
dc.subject.keywordPlusCELLS-
dc.subject.keywordPlusPROBES-
dc.subject.keywordPlusSTORM-
dc.subject.keywordPlusLIMIT-
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