Inertial Focusing of Particles in a Microfluidic Platform for Enhanced Discovery of High Affinity Peptide Sequences미세 유체 플랫폼에서 입자의 관성 집중 현상을 이용한 고결합력 펩타이드 발굴에 대한 연구

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Discovery of high affinity materials against a target of interest is crucial for various applications across multiple academic fields. Known as biopanning, the process enabled breakthroughs in targeted drug delivery and selective catalyst reduction. The use of phages displaying highly randomized peptide sequences on their coat proteins has been extensively researched. However, the efficient and controlled removal of non-bound and weakly-bound phages on the target material has been a challenge. By incorporating microfluidics, a controlled biopanning environment was enabled. Although current microfluidic biopanning systems have various advantages over conventional biopanning, the target material had to be fixed on a substrate. This meant that the wash flow around the target was limited to only the surface exposed to the fluid flow. By exposing all of the target surface, more efficient washing may be performed. Here, we introduce a method for improved biopanning through the use of inertial focusing and trapping of particles in microscale vortices. Inertial focusing allows for controlled manipulation of particles in the microfluidic channel. The subsequent trapping of particles in microscale vortices allows for continuous washing of the freely-floating particles. Through this technique, we showed efficient washing of phages displaying streptavidin binding peptide against particles displaying streptavidin on the surface. In addition, phages were modified with fluorescent dye for visualization during the experiments. Our work provides a basis for biopanning in a vortex chip and has potential to be used for biopanning using non-adherent cells such as circulating tumor cells and immune cells.
Advisors
Nam, Yoon Sungresearcher남윤성researcherLee, Won Heeresearcher이원희researcher
Description
한국과학기술원 :신소재공학과,
Publisher
한국과학기술원
Issue Date
2017
Identifier
325007
Language
eng
Description

학위논문(석사) - 한국과학기술원 : 신소재공학과, 2017.2,[ii, 29 p. :]

Keywords

M13 bacteriophage; phage display; biopanning; microfluidics; inertial focusing; microscale vortices; vortex chip; fluorescent modification; 박테리오파지; 바이오패닝; 미세유체; 관성 집중; 미세 소용돌이; 형광 변형

URI
http://hdl.handle.net/10203/243163
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=675287&flag=dissertation
Appears in Collection
MS-Theses_Master(석사논문)
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