DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, Chang Yeol | ko |
dc.contributor.author | Kim, Hyo Yong | ko |
dc.contributor.author | Kim, Soeun | ko |
dc.contributor.author | Park, Ki Soo | ko |
dc.contributor.author | Park, Hyun Gyu | ko |
dc.date.accessioned | 2018-05-24T02:43:12Z | - |
dc.date.available | 2018-05-24T02:43:12Z | - |
dc.date.created | 2018-05-14 | - |
dc.date.created | 2018-05-14 | - |
dc.date.issued | 2018-05 | - |
dc.identifier.citation | ANALYST, v.143, no.9, pp.2023 - 2028 | - |
dc.identifier.issn | 0003-2654 | - |
dc.identifier.uri | http://hdl.handle.net/10203/242348 | - |
dc.description.abstract | We herein describe a simple and sensitive strategy to detect a small molecule-protein interaction based on terminal protection-mediated exponential strand displacement amplification (eSDA). In principle, the small molecule linked to a DNA probe protects the DNA probe against the exonuclease I-catalyzed degradation after its binding to the corresponding target protein. The protected DNA probe then serves as a template to promote eSDA. Consequently, a large number of duplexes are produced, which leads to a high fluorescence from a double-stranded DNA specific fluorescent dye, SYBR Green I. As a model system to prove this sensing strategy, the interaction between biotin and streptavidin (SA), which is known to be the strongest among the non-covalent biological interactions, was selected and its analytical performance was thoroughly investigated. As a result, SA was sensitively detected with the limit of detection of 16 pM. In addition, the practical applicability of this method was successfully demonstrated by reliably determining the SA in human serum. | - |
dc.language | English | - |
dc.publisher | ROYAL SOC CHEMISTRY | - |
dc.subject | FOLATE-BINDING-PROTEIN | - |
dc.subject | BIOTIN-STREPTAVIDIN INTERACTION | - |
dc.subject | LINKED DNA | - |
dc.subject | EXONUCLEASE III | - |
dc.subject | SIGNAL AMPLIFICATION | - |
dc.subject | FLUORESCENCE DETECTION | - |
dc.subject | QUANTUM DOTS | - |
dc.subject | ASSAY | - |
dc.subject | BIOSENSOR | - |
dc.subject | RECEPTOR | - |
dc.title | A simple and sensitive detection of small molecule-protein interactions based on terminal protection-mediated exponential strand displacement amplification | - |
dc.type | Article | - |
dc.identifier.wosid | 000431109400007 | - |
dc.identifier.scopusid | 2-s2.0-85046302904 | - |
dc.type.rims | ART | - |
dc.citation.volume | 143 | - |
dc.citation.issue | 9 | - |
dc.citation.beginningpage | 2023 | - |
dc.citation.endingpage | 2028 | - |
dc.citation.publicationname | ANALYST | - |
dc.identifier.doi | 10.1039/c8an00099a | - |
dc.contributor.localauthor | Park, Hyun Gyu | - |
dc.contributor.nonIdAuthor | Kim, Soeun | - |
dc.contributor.nonIdAuthor | Park, Ki Soo | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | FOLATE-BINDING-PROTEIN | - |
dc.subject.keywordPlus | BIOTIN-STREPTAVIDIN INTERACTION | - |
dc.subject.keywordPlus | LINKED DNA | - |
dc.subject.keywordPlus | EXONUCLEASE III | - |
dc.subject.keywordPlus | SIGNAL AMPLIFICATION | - |
dc.subject.keywordPlus | FLUORESCENCE DETECTION | - |
dc.subject.keywordPlus | QUANTUM DOTS | - |
dc.subject.keywordPlus | ASSAY | - |
dc.subject.keywordPlus | BIOSENSOR | - |
dc.subject.keywordPlus | RECEPTOR | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.