DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | Shin, Eui-Cheol | - |
dc.contributor.advisor | 신의철 | - |
dc.contributor.advisor | Park, Su-Hyung | - |
dc.contributor.advisor | 박수형 | - |
dc.contributor.author | Choi, Young Joon | - |
dc.contributor.author | 최영준 | - |
dc.date.accessioned | 2018-05-23T19:36:49Z | - |
dc.date.available | 2018-05-23T19:36:49Z | - |
dc.date.issued | 2017 | - |
dc.identifier.uri | http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=675793&flag=dissertation | en_US |
dc.identifier.uri | http://hdl.handle.net/10203/241980 | - |
dc.description | 학위논문(박사) - 한국과학기술원 : 의과학대학원, 2017.2,[vi, 71 p. :] | - |
dc.description.abstract | Interleukin (IL)-15 is a one of the cytokines sharing common gamma-chain receptor and has a critical role in maintaining basal proliferation of memory $CD8^+$ T cells. However, the response of IL-15 in-duced proliferation varies in different memory subsets of $CD8^+$ T cells and the mechanism is remained to be elucidated. In the present study, we examined IL-15-induced proliferation of memory $CD8^+$ T cells and related mechanisms with human CD45RA re-expressing memory $CD8^+$ T cells ($CD8^+$ Temra cells). First, $CD8^+$ Temra cells were rechecked to have terminally differentiated, senescent characteristics by phenotype analysis and telomere length assessment. Then, I demonstrated that sorted $CD8^+$ Temra cells have enhanced proliferation and cell cycle entry upon IL-15 stimulation in comparison with the oth-er memory $CD8^+$ T cells. Using MHC class I pentamers and in vitro IL-15 stimulation experiments, I found that IL-15-induced proliferation was inversely correlated with CD5 expression, and $CD8^+$ Temra cells express low level of CD5 on their cell surface. Sorted $CD5^{low}$ memory $CD8^+$ T cells exhibited enhanced IL-15-induced proliferation and both blocking and knocking down of CD5 also showed enhanced IL-15-induced proliferation of memory $CD8^+$ T cells indicating that CD5 regulate IL-15-induced proliferation. Then, I revealed that the blockade of CD5 signaling results in increased activation of mechanistic target of rapamycin (mTOR) pathway which is responsible for IL-15-induced proliferation. These results indicate that IL-15-induced proliferation is suppressed by CD5 which inhibits mTOR pathway and that $CD8^+$ Temra cells have increased IL-15-induced proliferation by expressing low level of CD5. | - |
dc.language | eng | - |
dc.publisher | 한국과학기술원 | - |
dc.subject | CD8 T cells | - |
dc.subject | Interleukin-15 | - |
dc.subject | CD5 | - |
dc.subject | Proliferation | - |
dc.subject | Mechanistic target of rapamycin | - |
dc.subject | CD8 T 세포 | - |
dc.subject | 인터루킨 15 | - |
dc.subject | 증식 | - |
dc.subject | mTOR | - |
dc.title | Mechanisms underlying enhanced IL-15-induced proliferation of CD45RA Re-Expressing $CD8^+$ T cells | - |
dc.title.alternative | CD45RA를 재발현하는 CD8 T세포의 인터루킨 15에 의한 증식반응 항진에 대한 기전 연구 | - |
dc.type | Thesis(Ph.D) | - |
dc.identifier.CNRN | 325007 | - |
dc.description.department | 한국과학기술원 :의과학대학원, | - |
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