DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kang, Kyung-Tae | ko |
dc.contributor.author | Kang, Gyu-Min | ko |
dc.contributor.author | Lee, Bong Soo | ko |
dc.contributor.author | Choi, In-Sung | ko |
dc.contributor.author | NAM, Yoon-Key | ko |
dc.date.accessioned | 2011-06-27T02:24:20Z | - |
dc.date.available | 2011-06-27T02:24:20Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2010-04 | - |
dc.identifier.citation | CHEMISTRY-AN ASIAN JOURNAL, v.5, no.8, pp.1804 - 1809 | - |
dc.identifier.issn | 1861-4728 | - |
dc.identifier.uri | http://hdl.handle.net/10203/24183 | - |
dc.description.abstract | The utilization of non-bio-fouling poly(oligo(ethylene glycol) methacrylate) (pOEGMA) films as a background material for the generation of neuronal patterns is reported here. Our previously reported method, which was surface-initiated, atom transfer radical polymerization of OEGMA, and subsequent activation of terminal hydroxyl groups of pOEGMA with disuccinimidyl carbonate, was employed for the generation of activated pOEGMA films on glass. Poly-L-lysine was then microcontact-printed onto the activated polymer films, followed by backfilling with poly(ethylene glycol) moieties. E18 hippocampal neurons were cultured on the chemically patterned substrate, and the resulting neuronal networks were analyzed by phase-contrast microscopy and whole-cell patch clamp method. The results indicated that the pOEGMA films played an important role in the generation of good-quality neuronal patterns for up to two weeks without any negative effects to neurons. | - |
dc.description.sponsorship | We thank Alexander Shalek of the Department of Chemistry and Chemical Biology of Harvard University for the patch clamp analysis. I.S.C. was grateful to the LG Yonam Foundation and K.K. to the BK21 program for financial support. This research was also supported by the National Research Foundation of Korea funded by the Ministry of Education, Science and Technology (2009-0083525 and 2009-0080081), Chung Moon Soul Center for Bio-information and Electronics in KAIST, and Industrial Source Technology Development Programs by Ministry of Knowledge Economy in Korea. | en |
dc.language | English | - |
dc.language.iso | en_US | en |
dc.publisher | WILEY-V C H VERLAG GMBH | - |
dc.title | Generation of Patterned Neuronal Networks on Cell-Repellant Poly(oligo(ethylene glycol) Methacrylate) Films | - |
dc.type | Article | - |
dc.identifier.wosid | 000281025200011 | - |
dc.identifier.scopusid | 2-s2.0-77955178093 | - |
dc.type.rims | ART | - |
dc.citation.volume | 5 | - |
dc.citation.issue | 8 | - |
dc.citation.beginningpage | 1804 | - |
dc.citation.endingpage | 1809 | - |
dc.citation.publicationname | CHEMISTRY-AN ASIAN JOURNAL | - |
dc.identifier.doi | 10.1002/asia.200900761 | - |
dc.embargo.liftdate | 9999-12-31 | - |
dc.embargo.terms | 9999-12-31 | - |
dc.contributor.localauthor | Choi, In-Sung | - |
dc.contributor.localauthor | NAM, Yoon-Key | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | biological activity | - |
dc.subject.keywordAuthor | cell adhesion | - |
dc.subject.keywordAuthor | neurochemistry | - |
dc.subject.keywordAuthor | polymers | - |
dc.subject.keywordAuthor | surface chemistry | - |
dc.subject.keywordPlus | TRANSFER RADICAL POLYMERIZATION | - |
dc.subject.keywordPlus | POLY(ETHYLENE GLYCOL) | - |
dc.subject.keywordPlus | HIPPOCAMPAL-NEURONS | - |
dc.subject.keywordPlus | DEFINED GEOMETRY | - |
dc.subject.keywordPlus | SILICON-OXIDE | - |
dc.subject.keywordPlus | SURFACES | - |
dc.subject.keywordPlus | GOLD | - |
dc.subject.keywordPlus | IMMOBILIZATION | - |
dc.subject.keywordPlus | BIOMOLECULES | - |
dc.subject.keywordPlus | ENHANCEMENT | - |
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