DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | Helfman, David M. | - |
dc.contributor.advisor | 헬프만, 데이비드 | - |
dc.contributor.author | Jeong, SukYeong | - |
dc.contributor.author | 정숙영 | - |
dc.date.accessioned | 2018-05-23T19:34:31Z | - |
dc.date.available | 2018-05-23T19:34:31Z | - |
dc.date.issued | 2016 | - |
dc.identifier.uri | http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=669276&flag=dissertation | en_US |
dc.identifier.uri | http://hdl.handle.net/10203/241836 | - |
dc.description | 학위논문(박사) - 한국과학기술원 : 생명과학과, 2016.2,[iv, 51 p. :] | - |
dc.description.abstract | Tropomyosins (Tpm) are a family of actin filament binding proteins that regulates actin cytoskeleton organization and various cellular functions. Alterations in Tpm expression are implicated in various properties of transformed cells. Here I report the identification and characterization of a novel high molecular weight isoform of tropomyosin, Tpm4.1, expressed from the human TPM4 gene. Tpm4.1 expression is down-regulated in breast cancer cells compared with untransformed breast epithelial cells and in highly metastatic breast cancer cell lines derived from poorly metastatic MDA-MD-231 cells. In addition, patients with invasive ductal breast carcinoma show decreased TPM4 expression compared with patients with ductal breast carcinoma in situ, and low TPM4 expression is associated with a poor prognosis. To gain insights into the role of Tpm4.1 in breast cancer, I inhibited its function using siRNA in untransformed breast epithelial cells, MCF10A, and the human breast cancer cell line MDA-MB-231. Loss of Tpm4.1 in MCF10A cells increases cell migration in wound-healing and Boyden chamber assays and invasion out of spheroids as well as disruption of cell-cell adhesions. Down-regulation of Tpm4.1 in poorly metastatic MDA-MB-231 cells led to less stress fibers and increased cell invasion and dissemination from spheroids into collagen gels. Collectively these findings suggest that decreased expression of Tpm4.1 might play a critical role during tumor progression by facilitating the metastatic potential of tumor cells. | - |
dc.language | eng | - |
dc.publisher | 한국과학기술원 | - |
dc.subject | tropomyosin | - |
dc.subject | TPM4 | - |
dc.subject | migration | - |
dc.subject | invasion | - |
dc.subject | cell adhesions | - |
dc.subject | 트로포미오신 | - |
dc.subject | 세포이동 | - |
dc.subject | 세포침윤 | - |
dc.subject | 세포부착 | - |
dc.title | Characterization of a novel high molecular weight tropomyosin Tpm4.1 involved in invasive behaviors in breast epithelial cells | - |
dc.title.alternative | 트로포미오신 Tpm4.1이 인간유방세포의 침윤적 성질에 미치는 역할 | - |
dc.type | Thesis(Ph.D) | - |
dc.identifier.CNRN | 325007 | - |
dc.description.department | 한국과학기술원 :생명과학과, | - |
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