DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | Heo, Won Do | - |
dc.contributor.advisor | 허원도 | - |
dc.contributor.author | Nguyen, Trang Thi Thu | - |
dc.contributor.author | TRANG | - |
dc.date.accessioned | 2018-05-23T19:34:27Z | - |
dc.date.available | 2018-05-23T19:34:27Z | - |
dc.date.issued | 2016 | - |
dc.identifier.uri | http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=669272&flag=dissertation | en_US |
dc.identifier.uri | http://hdl.handle.net/10203/241832 | - |
dc.description | 학위논문(박사) - 한국과학기술원 : 생명과학과, 2016.2,[ix, 89 p. :] | - |
dc.description.abstract | Cells migrate by directing Rac1 and Cdc42 activities as well as actin polymerization towards their front. Previous studies proposed that this polarization process requires a local positive feed-back in the front involving Rac small GTPase and actin polymerization with PI3K likely playing a coor-dinating role. Here we show that PLEKHG3 is a PI3K-regulated RhoGEF for Rac1 and Cdc42 that se-lectively binds to newly polymerized actin in the front of migrating fibroblasts. Optogenetic inactiva-tion of PLEKHG3 showed that PLEKHG3 is indispensable not only for inducing but also for maintain-ing cell polarity. By selectively binding to newly polymerized actin, PLEKHG3 promotes local Rac1/ Cdc42 activation to induce more local actin polymerization which in turn promotes recruitment of more PLEKHG3 to induce and maintain frontness. Thus, autocatalytic reinforcement of PLEKHG3 localiza-tion to the front provides a molecular basis for the proposed positive feedback loop that is required for cell polarization and directed migration. | - |
dc.language | eng | - |
dc.publisher | 한국과학기술원 | - |
dc.subject | PLEKHG3 | - |
dc.subject | directed migration | - |
dc.subject | optogenetic | - |
dc.subject | positive feedback | - |
dc.subject | Rac1 | - |
dc.title | PLEKHG3 guides directed cell migration by activating branched actin networks | - |
dc.title.alternative | PLEKHG3의 액틴미세섬유 네트워크 활성을 통한 세포이동 메커니즘에 대한 연구 | - |
dc.type | Thesis(Ph.D) | - |
dc.identifier.CNRN | 325007 | - |
dc.description.department | 한국과학기술원 :생명과학과, | - |
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