DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lim, Mi Hee | ko |
dc.date.accessioned | 2018-02-21T06:25:23Z | - |
dc.date.available | 2018-02-21T06:25:23Z | - |
dc.date.created | 2018-02-08 | - |
dc.date.created | 2018-02-08 | - |
dc.date.created | 2018-02-08 | - |
dc.date.created | 2018-02-08 | - |
dc.date.issued | 2007-03 | - |
dc.identifier.citation | NATURE PROTOCOLS, v.2, no.2, pp.408 - 415 | - |
dc.identifier.issn | 1754-2189 | - |
dc.identifier.uri | http://hdl.handle.net/10203/240328 | - |
dc.description.abstract | A procedure for the preparation of a copper(II) complex (CuFL) as a fluorescent nitric oxide ( NO) detector is described. The fluorescein-based ligand FL can be synthesized in seven reaction steps (overall yield similar to 20%), typically requiring a total time of 9 days. The CuFL probe allows for the detection of NO produced in mammalian cultured cells. The detailed protocol for the use of CuFL for imaging NO in human neuroblastoma SK-N-SH cells takes a total time of similar to 26 h. This includes plating cells on six-well tissue culture plates or imaging dishes, treatment with CuFL, stimulation of NO synthases and imaging by fluorescence microscopy. | - |
dc.language | English | - |
dc.publisher | NATURE PUBLISHING GROUP | - |
dc.title | Preparation of a copper-based fluorescent probe for nitric oxide and its use in mammalian cultured cells | - |
dc.type | Article | - |
dc.identifier.wosid | 000253138100022 | - |
dc.identifier.scopusid | 2-s2.0-34347250850 | - |
dc.type.rims | ART | - |
dc.citation.volume | 2 | - |
dc.citation.issue | 2 | - |
dc.citation.beginningpage | 408 | - |
dc.citation.endingpage | 415 | - |
dc.citation.publicationname | NATURE PROTOCOLS | - |
dc.identifier.doi | 10.1038/nprot.2007.43 | - |
dc.contributor.localauthor | Lim, Mi Hee | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | NOBEL LECTURE | - |
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