Effects of Clioquinol on Metal-Triggered Amyloid-beta Aggregation Revisited

Abstract

Amyloid-beta (A beta) plaques are largely associated with the neuropathogenesis of Alzheimer's disease (AD). Metal ions such as Cu(II) and Zn(II) have been implicated as contributors to their formation and deposition, Metal chelators have been used to modulate metal-induced A beta aggregation. The bidentate ligand clioquinol (CC) presents an effective influence on metal-involved A beta aggregation, which has been explained through its metal chelation and is generally monitored by fluorescence and turbidity assays in vitro. The studies herein, however, suggest that the effects of CO on metal-driven A beta aggregation may not be visualized accurately by both assays. Subsequently, the present work demonstrates that CQ is able to chelate metal ions from metal-A beta species and to assist, in part, in the disaggregation of A beta aggregates, but it could not completely hinder the progression of A beta aggregation,