Compensation of aberration in quantitative phase imaging using lateral shifting and spiral phase integration

Abstract

We present a simple and effective method to eliminate system aberrations in quantitative phase imaging. Using spiral phase integration, complete information about system aberration is calculated from three laterally shifted phase images. The present method is especially useful when measuring confluent samples in which acquisition of background area is challenging. To demonstrate validity and applicability, we present measurements of various types of samples including microspheres, HeLa cells, and mouse brain tissue. Working conditions and limitations are systematically analyzed and discussed. (C) 2017 Optical Society of America under the terms of the OSA Open Access Publishing Agreement