The present invention relates to a sealed high-density on-line measuring device for spectrophotometer whose cuvette holder is linked to a reactor, which comprises a base; a fixed plate for light passage, which is vertically placed on the base; a moving plate for light radiation, which is vertically placed on the base at an opposite side to the fixed plate so that it can slide on the base horizontally; inlet and outlet for sample passage, each of which is positioned at outer side of the moving plate; a metal ring linking the fixed plate and the moving plate; and, upper and lower shielders each of whose ends is in contact with the fixed plate and moving plate, respectively, to give a sealed space. In accordance with the scaled on-line measuring device of the invention, high concentrations of samples in solutions can be measured without dilution, by way of employing a newly designed cuvette holder in a spectrophotometer fitted to a reactor.