DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, Sang-Yup | ko |
dc.contributor.author | Jeong, Ki-Jun | ko |
dc.date.accessioned | 2017-12-20T10:30:01Z | - |
dc.date.available | 2017-12-20T10:30:01Z | - |
dc.date.issued | 2006-07-04 | - |
dc.identifier.uri | http://hdl.handle.net/10203/232571 | - |
dc.description.abstract | The present invention provides a recombinant plasmid vector comprising a kanamycin resistance gene, a promoter, an endoxylanase signal sequence, a nucleotide sequence coding for an oligopeptide consisting of 13 amino acids including 6 consecutive histidine residues, and a human granulocyte colony stimulating factor(hG-CSF) gene; an E. coli transformed with the said vector; and, a process for producing complete hG-CSF protein with high purity from the protein pool secreted by the said microorganism. In accordance with the invention, the hG-CSF protein can be prepared with high purity through rather simple process facilitating secretion of large amount of hG-CSF fusion protein into the periplasm, which does not require complicated processes such as solubilization and subsequent refolding required for isolation of the hG-CSF protein produced in cytoplasm as insoluble inclusion bodies by conventional techniques, thus, the hG-CSF protein can be widely used as an active ingredient in the development of supplementary agents for anticancer therapy. | - |
dc.title | Escherichia coli strain secreting human granulocyte colony stimulating factor (G-CSF) | - |
dc.title.alternative | 인체 과립성 백혈구의 콜로니 자극인자를 분비하는 대장균 | - |
dc.type | Patent | - |
dc.type.rims | PAT | - |
dc.contributor.localauthor | Lee, Sang-Yup | - |
dc.contributor.nonIdAuthor | Jeong, Ki-Jun | - |
dc.contributor.assignee | KAIST | - |
dc.identifier.iprsType | 특허 | - |
dc.identifier.patentApplicationNumber | 10009792 | - |
dc.identifier.patentRegistrationNumber | 7070989 | - |
dc.date.application | 2001-03-31 | - |
dc.date.registration | 2006-07-04 | - |
dc.publisher.country | US | - |
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