DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Hee Yeon | ko |
dc.contributor.author | Kang, Jung Ae | ko |
dc.contributor.author | Ryou, Jeong-Hyun | ko |
dc.contributor.author | Lee, Gyeong Hee | ko |
dc.contributor.author | Choi, Dae Seong | ko |
dc.contributor.author | Lee, Dong Eun | ko |
dc.contributor.author | Kim, Hak-Sung | ko |
dc.date.accessioned | 2017-12-19T03:02:00Z | - |
dc.date.available | 2017-12-19T03:02:00Z | - |
dc.date.created | 2017-12-11 | - |
dc.date.created | 2017-12-11 | - |
dc.date.issued | 2017-11 | - |
dc.identifier.citation | ACS CHEMICAL BIOLOGY, v.12, no.11, pp.2891 - 2897 | - |
dc.identifier.issn | 1554-8929 | - |
dc.identifier.uri | http://hdl.handle.net/10203/228590 | - |
dc.description.abstract | With the high efficacy of protein-based therapeutics and plenty of intracellular drug targets, cytosolic protein delivery in a cell-specific manner has attracted considerable attention in the field of precision medicine. Herein, we present an intracellular protein delivery system based on a target-specific repebody and the translocation domain of Pseudomonas aeruginosa exotoxin A. The delivery platform was constructed by genetically fusing an EGFR-specific repebody as a targeting moiety to the translocation domain, while a protein cargo was fused to the C-terminal end of the delivery platform. The delivery platform was revealed to efficiently translocate a protein cargo to the cytosol in a target-specific manner. We demonstrate the utility and potential of the delivery platform by showing a remarkable tumor regression with negligible toxicity in a xenograft mice model when gelonin was used as the cytotoxic protein cargo. The present platform can find wide applications to the cell-selective cytosolic delivery of diverse proteins in many areas. | - |
dc.language | English | - |
dc.publisher | AMER CHEMICAL SOC | - |
dc.subject | IN-VITRO | - |
dc.subject | DRUG-DELIVERY | - |
dc.subject | CANCER | - |
dc.subject | TOXIN | - |
dc.subject | VIVO | - |
dc.subject | EGFR | - |
dc.subject | ANTIBODY | - |
dc.subject | THERAPY | - |
dc.subject | GELONIN | - |
dc.subject | DESIGN | - |
dc.title | Intracellular Protein Delivery System Using a Target-Specific Repebody and Translocation Domain of Bacterial Exotoxin | - |
dc.type | Article | - |
dc.identifier.wosid | 000416204500024 | - |
dc.identifier.scopusid | 2-s2.0-85034630339 | - |
dc.type.rims | ART | - |
dc.citation.volume | 12 | - |
dc.citation.issue | 11 | - |
dc.citation.beginningpage | 2891 | - |
dc.citation.endingpage | 2897 | - |
dc.citation.publicationname | ACS CHEMICAL BIOLOGY | - |
dc.identifier.doi | 10.1021/acschembio.7b00562 | - |
dc.contributor.localauthor | Kim, Hak-Sung | - |
dc.contributor.nonIdAuthor | Kang, Jung Ae | - |
dc.contributor.nonIdAuthor | Lee, Gyeong Hee | - |
dc.contributor.nonIdAuthor | Choi, Dae Seong | - |
dc.contributor.nonIdAuthor | Lee, Dong Eun | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | IN-VITRO | - |
dc.subject.keywordPlus | DRUG-DELIVERY | - |
dc.subject.keywordPlus | CANCER | - |
dc.subject.keywordPlus | TOXIN | - |
dc.subject.keywordPlus | VIVO | - |
dc.subject.keywordPlus | EGFR | - |
dc.subject.keywordPlus | ANTIBODY | - |
dc.subject.keywordPlus | THERAPY | - |
dc.subject.keywordPlus | GELONIN | - |
dc.subject.keywordPlus | DESIGN | - |
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