DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Hee Ja | ko |
dc.contributor.author | Lim, Jiwoo | ko |
dc.contributor.author | Jang, Young-Soon | ko |
dc.contributor.author | Shin, Eui-Cheol | ko |
dc.contributor.author | Kim, Hyung-Ran | ko |
dc.contributor.author | Seoh, Ju-Young | ko |
dc.contributor.author | Lee, Ji Soo | ko |
dc.contributor.author | Lee, Soon Nam | ko |
dc.contributor.author | Kang, Jihee Lee | ko |
dc.contributor.author | Choi, Youn-Hee | ko |
dc.date.accessioned | 2017-12-19T01:23:36Z | - |
dc.date.available | 2017-12-19T01:23:36Z | - |
dc.date.created | 2017-12-04 | - |
dc.date.created | 2017-12-04 | - |
dc.date.issued | 2017 | - |
dc.identifier.citation | CELLULAR PHYSIOLOGY AND BIOCHEMISTRY, v.42, no.6, pp.2467 - 2480 | - |
dc.identifier.issn | 1015-8987 | - |
dc.identifier.uri | http://hdl.handle.net/10203/228538 | - |
dc.description.abstract | Background/Aims: CD4+ T cells are a critical component of the adaptive immune response. While the mechanisms controlling the differentiation of the Th1, Th17, and regulatory T cell subsets from naive CD4+ T cells are well described, the factors that induce Th2 differentiation are still largely unknown. Methods: The effects of treatment with exogenous H2O2 on STAT-6 phosphorylation and activation in T cells were examined by imnnunoblotting, imnnunofluorescence and gel shift assay. Anti-CD3 antibody and methyl-beta-cyclodextrin were utilized to induce lipid raft assembly and to investigate the involvement of lipid rafts, respectively. Results: Jurkat and EL-4T cells that were exposed to H2O2 showed rapid and strong STAT-6 phosphorylation, and the extent of STAT-6 phosphorylation was enhanced by co-treatment with anti-CD3 antibody. The effect of H2O2 on STAT-6 phosphorylation and translocation was inhibited by disruption of lipid rafts. STAT-6 activation in response to H2O2 treatment regulated IL-4 gene expression, and this response was strengthened by treatment with anti-CD3. Conclusion: Our results indicate that reactive oxygen species such as H2O2 can act on upstream and initiating factors for activation of STAT-6 in T cells and contribute to formation of a positive feedback loop between STAT-6 and IL-4 in the Th2 differentiation process. (C) 2017 The Author(s) Published by S. Karger AG, Basel | - |
dc.language | English | - |
dc.publisher | KARGER | - |
dc.subject | KAPPA-B ACTIVATION | - |
dc.subject | RECEPTOR | - |
dc.subject | INFLAMMATION | - |
dc.subject | RECRUITMENT | - |
dc.subject | ASTROCYTES | - |
dc.subject | TYPE-2 | - |
dc.subject | POLARIZATION | - |
dc.subject | LYMPHOCYTES | - |
dc.subject | MECHANISMS | - |
dc.subject | EXPRESSION | - |
dc.title | Exogenous Hydrogen Peroxide Induces Lipid Raft-Mediated STAT-6 Activation in T Cells | - |
dc.type | Article | - |
dc.identifier.wosid | 000415239800027 | - |
dc.identifier.scopusid | 2-s2.0-85028448363 | - |
dc.type.rims | ART | - |
dc.citation.volume | 42 | - |
dc.citation.issue | 6 | - |
dc.citation.beginningpage | 2467 | - |
dc.citation.endingpage | 2480 | - |
dc.citation.publicationname | CELLULAR PHYSIOLOGY AND BIOCHEMISTRY | - |
dc.identifier.doi | 10.1159/000480210 | - |
dc.contributor.localauthor | Shin, Eui-Cheol | - |
dc.contributor.nonIdAuthor | Kim, Hee Ja | - |
dc.contributor.nonIdAuthor | Lim, Jiwoo | - |
dc.contributor.nonIdAuthor | Jang, Young-Soon | - |
dc.contributor.nonIdAuthor | Kim, Hyung-Ran | - |
dc.contributor.nonIdAuthor | Seoh, Ju-Young | - |
dc.contributor.nonIdAuthor | Lee, Ji Soo | - |
dc.contributor.nonIdAuthor | Lee, Soon Nam | - |
dc.contributor.nonIdAuthor | Kang, Jihee Lee | - |
dc.contributor.nonIdAuthor | Choi, Youn-Hee | - |
dc.description.isOpenAccess | Y | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | Reactive oxygen species (ROS) | - |
dc.subject.keywordAuthor | IL-4 | - |
dc.subject.keywordAuthor | STAT-6 | - |
dc.subject.keywordAuthor | Lipid rafts | - |
dc.subject.keywordAuthor | Th2 cells | - |
dc.subject.keywordPlus | KAPPA-B ACTIVATION | - |
dc.subject.keywordPlus | RECEPTOR | - |
dc.subject.keywordPlus | INFLAMMATION | - |
dc.subject.keywordPlus | RECRUITMENT | - |
dc.subject.keywordPlus | ASTROCYTES | - |
dc.subject.keywordPlus | TYPE-2 | - |
dc.subject.keywordPlus | POLARIZATION | - |
dc.subject.keywordPlus | LYMPHOCYTES | - |
dc.subject.keywordPlus | MECHANISMS | - |
dc.subject.keywordPlus | EXPRESSION | - |
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