DC Field | Value | Language |
---|---|---|
dc.contributor.author | Woo, Je Wook | ko |
dc.contributor.author | Kim, Jungeun | ko |
dc.contributor.author | Il Kwon, Soon | ko |
dc.contributor.author | Corvalan, Claudia | ko |
dc.contributor.author | Cho, Seung Woo | ko |
dc.contributor.author | Kim, Hyeran | ko |
dc.contributor.author | Kim, Sang-Gyu | ko |
dc.contributor.author | Kim, Sang-Tae | ko |
dc.contributor.author | Choe, Sunghwa | ko |
dc.contributor.author | Kim, Jin-Soo | ko |
dc.date.accessioned | 2017-11-08T05:47:22Z | - |
dc.date.available | 2017-11-08T05:47:22Z | - |
dc.date.created | 2017-11-07 | - |
dc.date.created | 2017-11-07 | - |
dc.date.issued | 2015-11 | - |
dc.identifier.citation | NATURE BIOTECHNOLOGY, v.33, no.11, pp.1162 - U156 | - |
dc.identifier.issn | 1087-0156 | - |
dc.identifier.uri | http://hdl.handle.net/10203/226946 | - |
dc.description.abstract | Editing plant genomes without introducing foreign DNA into cells may alleviate regulatory concerns related to genetically modified plants. We transfected preassembled complexes of purified Cas9 protein and guide RNA into plant protoplasts of Arabidopsis thaliana, tobacco, lettuce and rice and achieved targeted mutagenesis in regenerated plants at frequencies of up to 46%. The targeted sites contained germline-transmissible small insertions or deletions that are indistinguishable from naturally occurring genetic variation. | - |
dc.language | English | - |
dc.publisher | NATURE PUBLISHING GROUP | - |
dc.subject | RNA-GUIDED ENDONUCLEASES | - |
dc.subject | TRANSIENT GENE-EXPRESSION | - |
dc.subject | HUMAN-CELLS | - |
dc.subject | CAS9 | - |
dc.subject | ARABIDOPSIS | - |
dc.subject | SYSTEM | - |
dc.subject | PROTOPLASTS | - |
dc.subject | NUCLEASES | - |
dc.subject | KNOCKOUT | - |
dc.title | DNA-free genome editing in plants with preassembled CRISPR-Cas9 ribonucleoproteins | - |
dc.type | Article | - |
dc.identifier.wosid | 000364916000023 | - |
dc.identifier.scopusid | 2-s2.0-84947255513 | - |
dc.type.rims | ART | - |
dc.citation.volume | 33 | - |
dc.citation.issue | 11 | - |
dc.citation.beginningpage | 1162 | - |
dc.citation.endingpage | U156 | - |
dc.citation.publicationname | NATURE BIOTECHNOLOGY | - |
dc.identifier.doi | 10.1038/nbt.3389 | - |
dc.contributor.localauthor | Kim, Sang-Gyu | - |
dc.contributor.nonIdAuthor | Woo, Je Wook | - |
dc.contributor.nonIdAuthor | Kim, Jungeun | - |
dc.contributor.nonIdAuthor | Il Kwon, Soon | - |
dc.contributor.nonIdAuthor | Corvalan, Claudia | - |
dc.contributor.nonIdAuthor | Cho, Seung Woo | - |
dc.contributor.nonIdAuthor | Kim, Hyeran | - |
dc.contributor.nonIdAuthor | Kim, Sang-Tae | - |
dc.contributor.nonIdAuthor | Choe, Sunghwa | - |
dc.contributor.nonIdAuthor | Kim, Jin-Soo | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | RNA-GUIDED ENDONUCLEASES | - |
dc.subject.keywordPlus | TRANSIENT GENE-EXPRESSION | - |
dc.subject.keywordPlus | HUMAN-CELLS | - |
dc.subject.keywordPlus | CAS9 | - |
dc.subject.keywordPlus | ARABIDOPSIS | - |
dc.subject.keywordPlus | SYSTEM | - |
dc.subject.keywordPlus | PROTOPLASTS | - |
dc.subject.keywordPlus | NUCLEASES | - |
dc.subject.keywordPlus | KNOCKOUT | - |
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