DC Field | Value | Language |
---|---|---|
dc.contributor.author | Yoo, Seung-Hee | ko |
dc.contributor.author | Kojima, Shihoko | ko |
dc.contributor.author | Shimomura, Kazuhiro | ko |
dc.contributor.author | Koike, Nobuya | ko |
dc.contributor.author | Buhr, Ethan D. | ko |
dc.contributor.author | Furukawa, Tadashi | ko |
dc.contributor.author | Ko, Caroline H. | ko |
dc.contributor.author | Gloston, Gabrielle | ko |
dc.contributor.author | Ayoub, Christopher | ko |
dc.contributor.author | Nohara, Kazunari | ko |
dc.contributor.author | Reyes, Bryan A. | ko |
dc.contributor.author | Tsuchiya, Yoshiki | ko |
dc.contributor.author | Yoo, Ook-Joon | ko |
dc.contributor.author | Yagita, Kazuhiro | ko |
dc.contributor.author | Lee, Choogon | ko |
dc.contributor.author | Chen, Zheng | ko |
dc.contributor.author | Yamazaki, Shin | ko |
dc.contributor.author | Green, Carla B. | ko |
dc.contributor.author | Takahashi, Joseph S. | ko |
dc.date.accessioned | 2017-11-08T05:46:02Z | - |
dc.date.available | 2017-11-08T05:46:02Z | - |
dc.date.created | 2017-11-06 | - |
dc.date.created | 2017-11-06 | - |
dc.date.issued | 2017-10 | - |
dc.identifier.citation | PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, v.114, no.42, pp.E8855 - E8864 | - |
dc.identifier.issn | 0027-8424 | - |
dc.identifier.uri | http://hdl.handle.net/10203/226916 | - |
dc.description.abstract | We previously created two PER2:: LUCIFERASE (PER2:: LUC) circadian reporter knockin mice that differ only in the Per2 3'-UTR region: Per2:: Luc, which retains the endogenous Per2 3'-UTR and Per2:: LucSV, where the endogenous Per2 3'-UTR was replaced by an SV40 late poly(A) signal. To delineate the in vivo functions of Per2 3'-UTR, we analyzed circadian rhythms of Per2:: LucSV mice. Interestingly, Per2:: LucSV mice displayed more than threefold stronger amplitude in bioluminescence rhythms than Per2:: Luc mice, and also exhibited lengthened freerunning periods (similar to 24.0 h), greater phase delays following light pulse, and enhanced temperature compensation relative to Per2:: Luc. Analysis of the Per2 3'-UTR sequence revealed that miR-24, and to a lesser degree miR-30, suppressed PER2 protein translation, and the reversal of this inhibition in Per2:: LucSV augmented PER2:: LUC protein level and oscillatory amplitude. Interestingly, Bmal1mRNA and protein oscillatory amplitude as well as CRY1 protein oscillation were increased in Per2:: LucSV mice, suggesting rhythmic overexpression of PER2 enhances expression of Per2 and other core clock genes. Together, these studies provide important mechanistic insights into the regulatory roles of Per2 3'-UTR, miR-24, and PER2 in Per2 expression and core clock function. | - |
dc.language | English | - |
dc.publisher | NATL ACAD SCIENCES | - |
dc.subject | GENE-EXPRESSION | - |
dc.subject | REVEALS PERSISTENT | - |
dc.subject | CLOCK GENE | - |
dc.subject | IN-VIVO | - |
dc.subject | TEMPERATURE | - |
dc.subject | OSCILLATORS | - |
dc.subject | DEGRADATION | - |
dc.subject | MECHANISM | - |
dc.subject | MPER2 | - |
dc.subject | BMAL1 | - |
dc.title | Period2 3 '-UTR and microRNA-24 regulate circadian rhythms by repressing PERIOD2 protein accumulation | - |
dc.type | Article | - |
dc.identifier.wosid | 000413237900012 | - |
dc.identifier.scopusid | 2-s2.0-85031319756 | - |
dc.type.rims | ART | - |
dc.citation.volume | 114 | - |
dc.citation.issue | 42 | - |
dc.citation.beginningpage | E8855 | - |
dc.citation.endingpage | E8864 | - |
dc.citation.publicationname | PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | - |
dc.identifier.doi | 10.1073/pnas.1706611114 | - |
dc.contributor.localauthor | Yoo, Ook-Joon | - |
dc.contributor.nonIdAuthor | Yoo, Seung-Hee | - |
dc.contributor.nonIdAuthor | Kojima, Shihoko | - |
dc.contributor.nonIdAuthor | Shimomura, Kazuhiro | - |
dc.contributor.nonIdAuthor | Koike, Nobuya | - |
dc.contributor.nonIdAuthor | Buhr, Ethan D. | - |
dc.contributor.nonIdAuthor | Furukawa, Tadashi | - |
dc.contributor.nonIdAuthor | Ko, Caroline H. | - |
dc.contributor.nonIdAuthor | Gloston, Gabrielle | - |
dc.contributor.nonIdAuthor | Ayoub, Christopher | - |
dc.contributor.nonIdAuthor | Nohara, Kazunari | - |
dc.contributor.nonIdAuthor | Reyes, Bryan A. | - |
dc.contributor.nonIdAuthor | Tsuchiya, Yoshiki | - |
dc.contributor.nonIdAuthor | Yagita, Kazuhiro | - |
dc.contributor.nonIdAuthor | Lee, Choogon | - |
dc.contributor.nonIdAuthor | Chen, Zheng | - |
dc.contributor.nonIdAuthor | Yamazaki, Shin | - |
dc.contributor.nonIdAuthor | Green, Carla B. | - |
dc.contributor.nonIdAuthor | Takahashi, Joseph S. | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | Per2 gene | - |
dc.subject.keywordAuthor | circadian | - |
dc.subject.keywordAuthor | miR-24 | - |
dc.subject.keywordAuthor | microRNA | - |
dc.subject.keywordAuthor | 3&apos | - |
dc.subject.keywordAuthor | -UTR regulation | - |
dc.subject.keywordPlus | GENE-EXPRESSION | - |
dc.subject.keywordPlus | REVEALS PERSISTENT | - |
dc.subject.keywordPlus | CLOCK GENE | - |
dc.subject.keywordPlus | IN-VIVO | - |
dc.subject.keywordPlus | TEMPERATURE | - |
dc.subject.keywordPlus | OSCILLATORS | - |
dc.subject.keywordPlus | DEGRADATION | - |
dc.subject.keywordPlus | MECHANISM | - |
dc.subject.keywordPlus | MPER2 | - |
dc.subject.keywordPlus | BMAL1 | - |
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