DC Field | Value | Language |
---|---|---|
dc.contributor.author | Park, Jong Hyun | ko |
dc.contributor.author | Choi, Min Ah | ko |
dc.contributor.author | Kim, Yong Jae | ko |
dc.contributor.author | Kim, Yeu-Chun | ko |
dc.contributor.author | Chang, Yong Keun | ko |
dc.contributor.author | Jeong, Ki Jun | ko |
dc.date.accessioned | 2017-11-08T05:04:02Z | - |
dc.date.available | 2017-11-08T05:04:02Z | - |
dc.date.created | 2017-10-30 | - |
dc.date.created | 2017-10-30 | - |
dc.date.created | 2017-10-30 | - |
dc.date.issued | 2017-12 | - |
dc.identifier.citation | BIORESOURCE TECHNOLOGY, v.245, pp.1386 - 1392 | - |
dc.identifier.issn | 0960-8524 | - |
dc.identifier.uri | http://hdl.handle.net/10203/226813 | - |
dc.description.abstract | The Klebsiella oxytoca was engineered to produce 2,3-butanediol (2,3-BDO) simultaneously utilizing glucose and galactose obtained from a Golenkinia sp. hydrolysate. For efficient uptake of galactose at a high concentration of glucose, Escherichia coli galactose permease (GalP) was introduced, and the expression of galP under a weak-strength promoter resulted in simultaneous consumption of galactose and glucose. Next, to improve the sugar consumption, a gene encoding methylglyoxal synthase (MgsA) known as an inhibitor of multisugar metabolism was deleted, and the mgsA-null mutant showed much faster consumption of both sugars than the wild-type strain did. Finally, we demonstrated that the engineered K. oxytoca could utilize sugar extracts from a Golenkinia sp. hydrolysate and successfully produces 2,3-BDO. (C) 2017 Elsevier Ltd. All rights reserved. | - |
dc.language | English | - |
dc.publisher | ELSEVIER SCI LTD | - |
dc.title | Engineering of Klebsiella oxytoca for production of 2,3-butanediol via simultaneous utilization of sugars from a Golenkinia sp hydrolysate | - |
dc.type | Article | - |
dc.identifier.wosid | 000413038300011 | - |
dc.identifier.scopusid | 2-s2.0-85020445781 | - |
dc.type.rims | ART | - |
dc.citation.volume | 245 | - |
dc.citation.beginningpage | 1386 | - |
dc.citation.endingpage | 1392 | - |
dc.citation.publicationname | BIORESOURCE TECHNOLOGY | - |
dc.identifier.doi | 10.1016/j.biortech.2017.05.111 | - |
dc.contributor.localauthor | Kim, Yeu-Chun | - |
dc.contributor.localauthor | Chang, Yong Keun | - |
dc.contributor.localauthor | Jeong, Ki Jun | - |
dc.contributor.nonIdAuthor | Choi, Min Ah | - |
dc.contributor.nonIdAuthor | Kim, Yong Jae | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | Klebsiella oxytoca | - |
dc.subject.keywordAuthor | Golenkinia sp. | - |
dc.subject.keywordAuthor | Galactose permease | - |
dc.subject.keywordAuthor | Microalgae hydrolysate | - |
dc.subject.keywordAuthor | 2,3-Butanediol | - |
dc.subject.keywordPlus | CARBON CATABOLITE REPRESSION | - |
dc.subject.keywordPlus | HIGH-LEVEL EXPRESSION | - |
dc.subject.keywordPlus | ESCHERICHIA-COLI | - |
dc.subject.keywordPlus | PROTEIN | - |
dc.subject.keywordPlus | GENE | - |
dc.subject.keywordPlus | METHYLGLYOXAL | - |
dc.subject.keywordPlus | PROMOTER | - |
dc.subject.keywordPlus | BACTERIA | - |
dc.subject.keywordPlus | ACID | - |
dc.subject.keywordPlus | MECHANISMS | - |
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