DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kang, Yoo Kyung | ko |
dc.contributor.author | Kwon, Kyu | ko |
dc.contributor.author | Ryu, Jea Sung | ko |
dc.contributor.author | Lee, Ha Neul | ko |
dc.contributor.author | Park, Chan-Kyu | ko |
dc.contributor.author | Chung, Hyun Jung | ko |
dc.date.accessioned | 2017-06-05T02:06:08Z | - |
dc.date.available | 2017-06-05T02:06:08Z | - |
dc.date.created | 2017-03-08 | - |
dc.date.created | 2017-03-08 | - |
dc.date.issued | 2017-04 | - |
dc.identifier.citation | BIOCONJUGATE CHEMISTRY, v.28, no.4, pp.957 - 967 | - |
dc.identifier.issn | 1043-1802 | - |
dc.identifier.uri | http://hdl.handle.net/10203/223860 | - |
dc.description.abstract | The overuse of antibiotics plays a major role in the emergence and spread of multidrug-resistant bacteria. A molecularly targeted, specific treatment method for bacterial pathogens can prevent this problem by reducing the selective pressure during microbial growth. Herein, we introduce a nonviral treatment strategy delivering genome editing material for targeting antibacterial resistance. We apply the CRISPR-Cas9 system, which has been recognized as an innovative tool for highly specific and efficient genome engineering in different organisms, as the delivery cargo. We utilize polymer-derivatized Cas9, by direct covalent modification of the protein with cationic polymer, for subsequent complexation with single-guide RNA targeting antibiotic resistance. We show that nanosized CRISPR complexes (= Cr-Nanocomplex) were successfully formed, while maintaining the functional activity of Cas9 endonuclease to induce double-strand DNA cleavage. We also demonstrate that the Cr-Nanocomplex designed to target mecA-the major gene involved in methicillin resistance-can be efficiently delivered into Methicillin-resistant Staphylococcus aureus (MRSA), and allow the editing of the bacterial genome with much higher efficiency compared to using native Cas9 complexes or conventional lipid-based formulations. The present study shows for the first time that a covalently modified CRISPR system allows nonviral, therapeutic genome editing, and can be potentially applied as a target specific antimicrobial. | - |
dc.language | English | - |
dc.publisher | AMER CHEMICAL SOC | - |
dc.subject | VIVO GENE DELIVERY | - |
dc.subject | IN-VIVO | - |
dc.subject | STAPHYLOCOCCUS-AUREUS | - |
dc.subject | VANCOMYCIN-RESISTANT | - |
dc.subject | EFFICIENT DELIVERY | - |
dc.subject | VIRAL VECTORS | - |
dc.subject | THERAPY | - |
dc.subject | POLYETHYLENIMINE | - |
dc.subject | NANOPARTICLES | - |
dc.subject | RNA | - |
dc.title | Nonviral Genome Editing Based on a Polymer-Derivatized CRISPR Nanocomplex for Targeting Bacterial Pathogens and Antibiotic Resistance | - |
dc.type | Article | - |
dc.identifier.wosid | 000399965800014 | - |
dc.identifier.scopusid | 2-s2.0-85018506781 | - |
dc.type.rims | ART | - |
dc.citation.volume | 28 | - |
dc.citation.issue | 4 | - |
dc.citation.beginningpage | 957 | - |
dc.citation.endingpage | 967 | - |
dc.citation.publicationname | BIOCONJUGATE CHEMISTRY | - |
dc.identifier.doi | 10.1021/acs.bioconjchem.6b00676 | - |
dc.contributor.localauthor | Park, Chan-Kyu | - |
dc.contributor.localauthor | Chung, Hyun Jung | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | VIVO GENE DELIVERY | - |
dc.subject.keywordPlus | IN-VIVO | - |
dc.subject.keywordPlus | STAPHYLOCOCCUS-AUREUS | - |
dc.subject.keywordPlus | VANCOMYCIN-RESISTANT | - |
dc.subject.keywordPlus | EFFICIENT DELIVERY | - |
dc.subject.keywordPlus | VIRAL VECTORS | - |
dc.subject.keywordPlus | THERAPY | - |
dc.subject.keywordPlus | POLYETHYLENIMINE | - |
dc.subject.keywordPlus | NANOPARTICLES | - |
dc.subject.keywordPlus | RNA | - |
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