DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, Joo Hyoung | ko |
dc.contributor.author | Lim, Sung-Min | ko |
dc.contributor.author | Park, Sun-Hye | ko |
dc.contributor.author | Min, Jeong-Ki | ko |
dc.contributor.author | Lee, Gyun Min | ko |
dc.contributor.author | Kim, Yeon-Gu | ko |
dc.date.accessioned | 2017-05-15T05:18:08Z | - |
dc.date.available | 2017-05-15T05:18:08Z | - |
dc.date.created | 2017-05-08 | - |
dc.date.created | 2017-05-08 | - |
dc.date.issued | 2017-04 | - |
dc.identifier.citation | PROCESS BIOCHEMISTRY, v.55, pp.182 - 186 | - |
dc.identifier.issn | 1359-5113 | - |
dc.identifier.uri | http://hdl.handle.net/10203/223665 | - |
dc.description.abstract | In an attempt to determine the relationship between the Epstein Barr virus nuclear antigen-1 (EBNA-1) expression level and specific foreign protein productivity (q(p)), EBNA-1-amplifed HEK293 cells, which achieved a higher EBNA-1 expression level than that achieved by HEK293E cells, were established using dihydrofolate reductase (dhfr)-mediated gene amplification. Compared with a control culture in a null pool, Fc-fusion protein production by transient transfection in the EBNA-1-amplified pool showed a significant improvement. qp was linearly correlated with the EBNA-1 expression level in the transient transfection of EBNA-1-amplified clones, as indicated by the correlation coefficient (R-2 = 0.7407). The Fc-fusion protein production and qp in a transient gene expression-based culture with EBNA-1-amplified HEK293 cells, E-amp-68, were approximately 2.0 and 3.2 times, respectively, higher than those in a culture with HEK293E cells. The increase in qp by EBNA-1 amplification mainly resulted from an enhancement in the amount of replicated DNA and level of mRNA expression but not an improved transfection efficiency. Taken together, it was found that EBNA-1 amplification could improve the therapeutic protein production in an HEK293 cell-based transient gene expression system. (C) 2017 Published by Elsevier Ltd. | - |
dc.language | English | - |
dc.publisher | ELSEVIER SCI LTD | - |
dc.subject | MAMMALIAN-CELLS | - |
dc.subject | ANTIBODY-PRODUCTION | - |
dc.subject | LARGE-SCALE | - |
dc.subject | CHO-CELLS | - |
dc.subject | TRANSGENE EXPRESSION | - |
dc.subject | VECTOR | - |
dc.subject | TRANSFECTION | - |
dc.subject | SYSTEM | - |
dc.subject | ENHANCEMENT | - |
dc.subject | PLASMID | - |
dc.title | Investigation of relationship between EBNA-1 expression level and specific foreign protein productivity in transient gene expression of HEK293 cells | - |
dc.type | Article | - |
dc.identifier.wosid | 000398878800021 | - |
dc.identifier.scopusid | 2-s2.0-85011574081 | - |
dc.type.rims | ART | - |
dc.citation.volume | 55 | - |
dc.citation.beginningpage | 182 | - |
dc.citation.endingpage | 186 | - |
dc.citation.publicationname | PROCESS BIOCHEMISTRY | - |
dc.identifier.doi | 10.1016/j.procbio.2017.01.020 | - |
dc.contributor.localauthor | Lee, Gyun Min | - |
dc.contributor.nonIdAuthor | Lim, Sung-Min | - |
dc.contributor.nonIdAuthor | Park, Sun-Hye | - |
dc.contributor.nonIdAuthor | Min, Jeong-Ki | - |
dc.contributor.nonIdAuthor | Kim, Yeon-Gu | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | HEK293 cells | - |
dc.subject.keywordAuthor | EBNA-1/oriP | - |
dc.subject.keywordAuthor | dhfr/MTX | - |
dc.subject.keywordAuthor | Transient gene expression | - |
dc.subject.keywordPlus | MAMMALIAN-CELLS | - |
dc.subject.keywordPlus | ANTIBODY-PRODUCTION | - |
dc.subject.keywordPlus | LARGE-SCALE | - |
dc.subject.keywordPlus | CHO-CELLS | - |
dc.subject.keywordPlus | TRANSGENE EXPRESSION | - |
dc.subject.keywordPlus | VECTOR | - |
dc.subject.keywordPlus | TRANSFECTION | - |
dc.subject.keywordPlus | SYSTEM | - |
dc.subject.keywordPlus | ENHANCEMENT | - |
dc.subject.keywordPlus | PLASMID | - |
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