DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Jung-Hee | ko |
dc.contributor.author | Sung, Pil Soo | ko |
dc.contributor.author | Lee, Eun B. | ko |
dc.contributor.author | Hur, Wonhee | ko |
dc.contributor.author | Park, Dong J. | ko |
dc.contributor.author | Shin, Eui-Cheol | ko |
dc.contributor.author | Windisch, Marc P. | ko |
dc.contributor.author | Yoon, Seung K. | ko |
dc.date.accessioned | 2017-05-15T05:17:54Z | - |
dc.date.available | 2017-05-15T05:17:54Z | - |
dc.date.created | 2017-05-08 | - |
dc.date.created | 2017-05-08 | - |
dc.date.issued | 2017-04 | - |
dc.identifier.citation | FRONTIERS IN MICROBIOLOGY, v.8 | - |
dc.identifier.issn | 1664-302X | - |
dc.identifier.uri | http://hdl.handle.net/10203/223660 | - |
dc.description.abstract | Gene-associated with retinoid-interferon-induced mortality 19 (GRIM-19) targets multiple signaling pathways involved in cell death and growth. However, the role of GRIM-19 in the pathogenesis of hepatitis virus infections remains unexplored. Here, we investigated the restrictive effects of GRIM-19 on the replication of hepatitis C virus (HCV). We found that GRIM-19 protein levels were reduced in HCV-infected Huh7 cells and Huh7 cells harboring HCV replicons. Moreover, ectopically expressed GRIM-19 caused a reduction in both intracellular viral RNA levels and secreted viruses in HCVcc-infected cell cultures. The restrictive effect on HCV replication was restored by treatment with siRNA against GRIM-19. Interestingly, GRIM-19 overexpression did not alter the level of phosphorylated STAT3 or its subcellular distribution. Strikingly, forced expression of GRIM-19 attenuated an increase in intracellular lipid droplets after oleic acid (OA) treatment or HCVcc infection. GRIM-19 overexpression abrogated fatty acid-induced upregulation of sterol regulatory element-binding transcription factor-1 (SREBP-1c), resulting in attenuated expression of its target genes such as fatty acid synthase (FAS) and acetyl CoA carboxylase (ACC). Treatment with OA or overexpression of SREBP-1c in GRIM-19-expressing, HCVcc-infected cells restored HCV replication. Our results suggest that GRIM-19 interferes with HCV replication by attenuating intracellular lipid accumulation and therefore is an anti-viral host factor that could be a promising target for HCV treatment. | - |
dc.language | English | - |
dc.publisher | FRONTIERS MEDIA SA | - |
dc.subject | HEPATITIS-C VIRUS | - |
dc.subject | ELEMENT-BINDING PROTEIN-1C | - |
dc.subject | VIRAL-RNA POLYMERASE | - |
dc.subject | CARCINOMA IN-VITRO | - |
dc.subject | INDUCED CELL-DEATH | - |
dc.subject | HEPATOCELLULAR-CARCINOMA | - |
dc.subject | STAT3 PATHWAY | - |
dc.subject | UP-REGULATION | - |
dc.subject | EXPRESSION | - |
dc.subject | LIPOGENESIS | - |
dc.title | GRIM-19 Restricts HCV Replication by Attenuating Intracellular Lipid Accumulation | - |
dc.type | Article | - |
dc.identifier.wosid | 000398768000001 | - |
dc.identifier.scopusid | 2-s2.0-85018268269 | - |
dc.type.rims | ART | - |
dc.citation.volume | 8 | - |
dc.citation.publicationname | FRONTIERS IN MICROBIOLOGY | - |
dc.identifier.doi | 10.3389/fmicb.2017.00576 | - |
dc.contributor.localauthor | Shin, Eui-Cheol | - |
dc.contributor.nonIdAuthor | Kim, Jung-Hee | - |
dc.contributor.nonIdAuthor | Lee, Eun B. | - |
dc.contributor.nonIdAuthor | Hur, Wonhee | - |
dc.contributor.nonIdAuthor | Park, Dong J. | - |
dc.contributor.nonIdAuthor | Windisch, Marc P. | - |
dc.contributor.nonIdAuthor | Yoon, Seung K. | - |
dc.description.isOpenAccess | Y | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | hepatitis C virus | - |
dc.subject.keywordAuthor | anti-viral host factor | - |
dc.subject.keywordAuthor | viral replication | - |
dc.subject.keywordAuthor | lipogenesis | - |
dc.subject.keywordAuthor | intracellular lipid accumulation | - |
dc.subject.keywordPlus | HEPATITIS-C VIRUS | - |
dc.subject.keywordPlus | ELEMENT-BINDING PROTEIN-1C | - |
dc.subject.keywordPlus | VIRAL-RNA POLYMERASE | - |
dc.subject.keywordPlus | CARCINOMA IN-VITRO | - |
dc.subject.keywordPlus | INDUCED CELL-DEATH | - |
dc.subject.keywordPlus | HEPATOCELLULAR-CARCINOMA | - |
dc.subject.keywordPlus | STAT3 PATHWAY | - |
dc.subject.keywordPlus | UP-REGULATION | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | LIPOGENESIS | - |
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