A dual substructuring method with a penalty term was introduced in the previous works by the authors, which is a variant of the FETI-DP method. The proposed method imposes the continuity not only by using Lagrange multipliers but also by adding a penalty term which consists of a positive penalty parameter n and a measure of the jump across the interface. Due to the penalty term, the proposed iterative method has a better convergence property than the standard FETI-DP method in the sense that the condition number of the resulting dual problem is bounded by a constant independent of the subdomain size and the mesh size. In this paper, a further study for a dual iterative substructuring method with a penalty term is discussed in terms of its convergence analysis. We provide an improved estimate of the condition number which shows the relationship between the condition number and eta as well as a close spectral connection of the proposed method with the FETI-DP method. As a result, a choice of a moderately small penalty parameter is guaranteed.