DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Jong Goo | ko |
dc.contributor.author | Muniyappan, Srinivasan | ko |
dc.contributor.author | Oang, Key Young | ko |
dc.contributor.author | Kim, Tae Wu | ko |
dc.contributor.author | Yang, Cheolhee | ko |
dc.contributor.author | Kim, Kyung Hwan | ko |
dc.contributor.author | Kim, Jeongho | ko |
dc.contributor.author | Ihee, Hyotcherl | ko |
dc.date.accessioned | 2017-04-17T06:43:21Z | - |
dc.date.available | 2017-04-17T06:43:21Z | - |
dc.date.created | 2016-06-08 | - |
dc.date.created | 2016-06-08 | - |
dc.date.issued | 2016-03 | - |
dc.identifier.citation | STRUCTURAL DYNAMICS, v.3, no.2 | - |
dc.identifier.issn | 2329-7778 | - |
dc.identifier.uri | http://hdl.handle.net/10203/223205 | - |
dc.description.abstract | Homodimeric hemoglobin (HbI) consisting of two subunits is a good model system for investigating the allosteric structural transition as it exhibits cooperativity in ligand binding. In this work, as an effort to extend our previous study on wild-type and F97Y mutant HbI, we investigate structural dynamics of a mutant HbI in solution to examine the role of well-organized interfacial water cluster, which has been known to mediate intersubunit communication in HbI. In the T72V mutant of HbI, the interfacial water cluster in the T state is perturbed due to the lack of Thr72, resulting in two less interfacial water molecules than in wild-type HbI. By performing picosecond time-resolved X-ray solution scattering experiment and kinetic analysis on the T72V mutant, we identify three structurally distinct intermediates (I-1, I-2, and I-3) and show that the kinetics of the T72V mutant are well described by the same kinetic model used for wild-type and F97Y HbI, which involves biphasic kinetics, geminate recombination, and bimolecular CO recombination. The optimized kinetic model shows that the R-T transition and bimolecular CO recombination are faster in the T72V mutant than in the wild type. From structural analysis using species-associated difference scattering curves for the intermediates, we find that the T-like deoxy I-3 intermediate in solution has a different structure from deoxy HbI in crystal. In addition, we extract detailed structural parameters of the intermediates such as E-F distance, intersubunit rotation angle, and heme-heme distance. By comparing the structures of protein intermediates in wild-type HbI and the T72V mutant, we reveal how the perturbation in the interfacial water cluster affects the kinetics and structures of reaction intermediates of HbI. (C) 2016 Author(s) | - |
dc.language | English | - |
dc.publisher | AMER INST PHYSICS | - |
dc.subject | SCAPHARCA DIMERIC HEMOGLOBIN | - |
dc.subject | FREE-ELECTRON LASER | - |
dc.subject | CRYSTAL-STRUCTURE | - |
dc.subject | LIGAND-BINDING | - |
dc.subject | ALLOSTERIC MECHANISM | - |
dc.subject | OXYGEN-BINDING | - |
dc.subject | INAEQUIVALVIS | - |
dc.subject | RESOLUTION | - |
dc.subject | TRANSITION | - |
dc.subject | RAMAN | - |
dc.title | Cooperative protein structural dynamics of homodimeric hemoglobin linked to water cluster at subunit interface revealed by time-resolved X-ray solution scattering | - |
dc.type | Article | - |
dc.identifier.wosid | 000375803100011 | - |
dc.identifier.scopusid | 2-s2.0-84966712910 | - |
dc.type.rims | ART | - |
dc.citation.volume | 3 | - |
dc.citation.issue | 2 | - |
dc.citation.publicationname | STRUCTURAL DYNAMICS | - |
dc.identifier.doi | 10.1063/1.4947071 | - |
dc.contributor.localauthor | Ihee, Hyotcherl | - |
dc.contributor.nonIdAuthor | Oang, Key Young | - |
dc.contributor.nonIdAuthor | Kim, Kyung Hwan | - |
dc.contributor.nonIdAuthor | Kim, Jeongho | - |
dc.description.isOpenAccess | Y | - |
dc.type.journalArticle | Article; Proceedings Paper | - |
dc.subject.keywordPlus | SCAPHARCA DIMERIC HEMOGLOBIN | - |
dc.subject.keywordPlus | FREE-ELECTRON LASER | - |
dc.subject.keywordPlus | CRYSTAL-STRUCTURE | - |
dc.subject.keywordPlus | LIGAND-BINDING | - |
dc.subject.keywordPlus | ALLOSTERIC MECHANISM | - |
dc.subject.keywordPlus | OXYGEN-BINDING | - |
dc.subject.keywordPlus | INAEQUIVALVIS | - |
dc.subject.keywordPlus | RESOLUTION | - |
dc.subject.keywordPlus | TRANSITION | - |
dc.subject.keywordPlus | RAMAN | - |
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