DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, Joong-jae | ko |
dc.contributor.author | Kang, Jung Ae | ko |
dc.contributor.author | Ryu, Yiseul | ko |
dc.contributor.author | Han, Sang-Soo | ko |
dc.contributor.author | Nam, You Ree | ko |
dc.contributor.author | Rho, Jong Kook | ko |
dc.contributor.author | Choi, Dae Seong | ko |
dc.contributor.author | Kang, Sun-Woong | ko |
dc.contributor.author | Lee, Dong-Eun | ko |
dc.contributor.author | Kim, Hak-Sung | ko |
dc.date.accessioned | 2017-03-30T09:17:37Z | - |
dc.date.available | 2017-03-30T09:17:37Z | - |
dc.date.created | 2017-03-29 | - |
dc.date.created | 2017-03-29 | - |
dc.date.issued | 2017-03 | - |
dc.identifier.citation | BIOMATERIALS, v.120, pp.22 - 31 | - |
dc.identifier.issn | 0142-9612 | - |
dc.identifier.uri | http://hdl.handle.net/10203/222697 | - |
dc.description.abstract | The integration of a targeted delivery with a tumour-selective agent has been considered an ideal platform for achieving high therapeutic efficacy and negligible side effects in cancer therapy. Here, we present engineered protein nanoparticles comprising a tumour-selective oncolytic protein and a targeting moiety as a new format for the targeted cancer therapy. Apoptin from chicken anaemia virus (CAV) was used as a tumour-selective apoptotic protein. An EGFR-specific repebody, which is composed of LRR (Leucine-rich repeat) modules, was employed to play a dual role as a tumour-targeting moiety and a fusion partner for producing apoptin nanoparticles in E. coli, respectively. The repebody was genetically fused to apoptin, and the resulting fusion protein was shown to self-assemble into supramolecular repebody-apoptin nanoparticles with high homogeneity and stability as a soluble form when expressed in E. coli. The repebody-apoptin nanoparticles showed a remarkable anti-tumour activity with negligible side effects in xenograft mice through a cooperative action of the two protein components with distinct functional roles. The repebody-apoptin nanoparticles can be developed as a systemic injectable and tumour-selective therapeutic protein for targeted cancer treatment. (C) 2016 Elsevier Ltd. All rights reserved. | - |
dc.language | English | - |
dc.publisher | ELSEVIER SCI LTD | - |
dc.subject | CHICKEN ANEMIA VIRUS | - |
dc.subject | TUMOR-CELLS | - |
dc.subject | IN-VITRO | - |
dc.subject | APOPTIN | - |
dc.subject | DELIVERY | - |
dc.subject | APOPTOSIS | - |
dc.subject | ANTIBODY | - |
dc.subject | DRUGS | - |
dc.subject | EGFR | - |
dc.subject | VIVO | - |
dc.title | Genetically engineered and self-assembled oncolytic protein nanoparticles for targeted cancer therapy | - |
dc.type | Article | - |
dc.identifier.wosid | 000394398900003 | - |
dc.identifier.scopusid | 2-s2.0-85007236130 | - |
dc.type.rims | ART | - |
dc.citation.volume | 120 | - |
dc.citation.beginningpage | 22 | - |
dc.citation.endingpage | 31 | - |
dc.citation.publicationname | BIOMATERIALS | - |
dc.identifier.doi | 10.1016/j.biomaterials.2016.12.014 | - |
dc.contributor.localauthor | Kim, Hak-Sung | - |
dc.contributor.nonIdAuthor | Kang, Jung Ae | - |
dc.contributor.nonIdAuthor | Nam, You Ree | - |
dc.contributor.nonIdAuthor | Rho, Jong Kook | - |
dc.contributor.nonIdAuthor | Choi, Dae Seong | - |
dc.contributor.nonIdAuthor | Kang, Sun-Woong | - |
dc.contributor.nonIdAuthor | Lee, Dong-Eun | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | Targeted delivery | - |
dc.subject.keywordAuthor | Cancer therapy | - |
dc.subject.keywordAuthor | Tumour selectivity | - |
dc.subject.keywordAuthor | Protein nanoparticle | - |
dc.subject.keywordAuthor | Repebody | - |
dc.subject.keywordAuthor | Self-assembly | - |
dc.subject.keywordPlus | CHICKEN ANEMIA VIRUS | - |
dc.subject.keywordPlus | TUMOR-CELLS | - |
dc.subject.keywordPlus | IN-VITRO | - |
dc.subject.keywordPlus | APOPTIN | - |
dc.subject.keywordPlus | DELIVERY | - |
dc.subject.keywordPlus | APOPTOSIS | - |
dc.subject.keywordPlus | ANTIBODY | - |
dc.subject.keywordPlus | DRUGS | - |
dc.subject.keywordPlus | EGFR | - |
dc.subject.keywordPlus | VIVO | - |
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