DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | Yoon, Tae-Young | - |
dc.contributor.advisor | 윤태영 | - |
dc.contributor.author | Yoo, Janghyun | - |
dc.contributor.author | 유장현 | - |
dc.date.accessioned | 2017-03-28T07:14:19Z | - |
dc.date.available | 2017-03-28T07:14:19Z | - |
dc.date.issued | 2016 | - |
dc.identifier.uri | http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=648119&flag=dissertation | en_US |
dc.identifier.uri | http://hdl.handle.net/10203/221126 | - |
dc.description | 학위논문(박사) - 한국과학기술원 : 물리학과, 2016.2 ,[vi, 50 p. :] | - |
dc.description.abstract | Weak and transient protein-protein interactions, that can show fast response to the cell environment change, are a key to understand cell signaling. However, their weakly interacting nature followed by high substrate concentration of sub-μM to hundreds of μM requirement in detection makes them hard study. Single-molecule imaging has high detection sensitivity. But diffraction limited observation volume makes its concentration barrier at nM range. Few approaches have successfully broken through the concentration barrier. However, in this study, instead of breaking the concentration barrier, we will bypass it through twisting the imaging scheme without major modification in conventional single-molecule imaging system. Increased bait density allowed us to monitor interactions at low prey concentration which is four orders of magnitude lower than its dissociation constant. It enabled us to quantify intertwined kinetics of signaling proteins: Ras, Raf and Raf dimers. This new scheme can be extended to study kinetics that requires tens of mM range or even further. | - |
dc.language | eng | - |
dc.publisher | 한국과학기술원 | - |
dc.subject | Single-molecule detection | - |
dc.subject | Single-molecule co-IP | - |
dc.subject | Protein-protein interaction | - |
dc.subject | Ras | - |
dc.subject | Raf | - |
dc.subject | 단분자 | - |
dc.subject | 단분자 공면역침강기법 | - |
dc.subject | 단백질 상호작용 | - |
dc.title | Single-molecule co-IP for quantitative protein-protein interaction analysis | - |
dc.title.alternative | 단분자 공면역침강기법을 이용한 정량적 단백질 상호작용 분석 | - |
dc.type | Thesis(Ph.D) | - |
dc.identifier.CNRN | 325007 | - |
dc.description.department | 한국과학기술원 :물리학과, | - |
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