DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Che Lin | ko |
dc.contributor.author | Ha, Tae Kwang | ko |
dc.contributor.author | Lee, Gyun Min | ko |
dc.date.accessioned | 2017-01-18T02:53:22Z | - |
dc.date.available | 2017-01-18T02:53:22Z | - |
dc.date.created | 2016-12-13 | - |
dc.date.created | 2016-12-13 | - |
dc.date.issued | 2016-09 | - |
dc.identifier.citation | BIOTECHNOLOGY AND BIOPROCESS ENGINEERING, v.21, no.5, pp.667 - 675 | - |
dc.identifier.issn | 1226-8372 | - |
dc.identifier.uri | http://hdl.handle.net/10203/219670 | - |
dc.description.abstract | Lithium chloride (LiCl), which induces cell cycle arrest at G2/M phase, is known as a specific production rate (q (p))-enhancing additive in recombinant Chinese hamster ovary (CHO) cell culture. To determine the potential of LiCl as a chemical additive that enhances transient gene expression (TGE), LiCl was added to the CHO-NK and human embryonic kidney 293E (HEK293E) cell cultures before and/or after transfection with polyethylenimine as a transfection reagent. The effect of this addition on transfection efficiency (pre-treatment) and q (p) enhancement during TGE (post-treatment) was examined. For the TGE of monoclonal antibody (mAb) in CHO-NK cells, pretreatment alone with 10 mM LiCl and post-treatment alone with 5 mM LiCl resulted in 1.2- and 3.4-fold increase of maximum mAb concentration (MMC), respectively, compared with the TGE without LiCl treatment. Furthermore, combinatorial treatment with LiCl (10 mM for pre-treatment and 5 mM for post-treatment) synergistically increased the TGE of mAb (5.3-fold increase in MMC). Likewise, combinatorial treatment with LiCl (10 mM for pre-treatment and 15 mM for post-treatment) in HEK293E cells synergistically increased the TGE of mAb (4.9-fold increase in MMC). Taken together, the data obtained here demonstrate that combinatorial treatment with LiCl is a useful means to improve TGE in CHO as well as HEK293 cells. | - |
dc.language | English | - |
dc.publisher | KOREAN SOC BIOTECHNOLOGY & BIOENGINEERING | - |
dc.subject | HAMSTER OVARY CELLS | - |
dc.subject | INTACT YEAST-CELLS | - |
dc.subject | FC-FUSION PROTEIN | - |
dc.subject | GENE-EXPRESSION | - |
dc.subject | VALPROIC ACID | - |
dc.subject | CULTURE | - |
dc.subject | TRANSFORMATION | - |
dc.subject | EFFICIENCY | - |
dc.subject | APOPTOSIS | - |
dc.subject | LINE | - |
dc.title | Combinatorial treatment with lithium chloride enhances recombinant antibody production in transiently transfected CHO and HEK293E cells | - |
dc.type | Article | - |
dc.identifier.wosid | 000387681800013 | - |
dc.identifier.scopusid | 2-s2.0-84994754076 | - |
dc.type.rims | ART | - |
dc.citation.volume | 21 | - |
dc.citation.issue | 5 | - |
dc.citation.beginningpage | 667 | - |
dc.citation.endingpage | 675 | - |
dc.citation.publicationname | BIOTECHNOLOGY AND BIOPROCESS ENGINEERING | - |
dc.identifier.doi | 10.1007/s12257-016-0434-8 | - |
dc.identifier.kciid | ART002163278 | - |
dc.contributor.localauthor | Lee, Gyun Min | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | cell cycle arrest | - |
dc.subject.keywordAuthor | CHO cells | - |
dc.subject.keywordAuthor | HEK293E cells | - |
dc.subject.keywordAuthor | lithium chloride | - |
dc.subject.keywordAuthor | transient gene expression | - |
dc.subject.keywordPlus | HAMSTER OVARY CELLS | - |
dc.subject.keywordPlus | INTACT YEAST-CELLS | - |
dc.subject.keywordPlus | FC-FUSION PROTEIN | - |
dc.subject.keywordPlus | GENE-EXPRESSION | - |
dc.subject.keywordPlus | VALPROIC ACID | - |
dc.subject.keywordPlus | CULTURE | - |
dc.subject.keywordPlus | TRANSFORMATION | - |
dc.subject.keywordPlus | EFFICIENCY | - |
dc.subject.keywordPlus | APOPTOSIS | - |
dc.subject.keywordPlus | LINE | - |
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