DC Field | Value | Language |
---|---|---|
dc.contributor.author | Choi, JH | ko |
dc.contributor.author | Seok Jae Lee | ko |
dc.contributor.author | Sang Jun Lee | ko |
dc.contributor.author | Lee, SangYup | ko |
dc.date.accessioned | 2011-01-28T05:16:35Z | - |
dc.date.available | 2011-01-28T05:16:35Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2003-08 | - |
dc.identifier.citation | APPLIED AND ENVIRONMENTAL MICROBIOLOGY, v.69, pp.4737 - 4742 | - |
dc.identifier.issn | 0099-2240 | - |
dc.identifier.uri | http://hdl.handle.net/10203/21894 | - |
dc.description.abstract | The transcriptome profiles of recombinant Escherichia coli producing human insulin-like growth factor I fusion protein (IGF-I-f) during the high-cell-density fed-batch culture were analyzed using DNA microarrays. The expression levels of 529 genes were significantly altered after induction. About 200 genes were significantly down-regulated during the production of IGF-I-f after induction. Among these down-regulated genes, we rationally selected and coexpressed in E. coli producing IGF-I-f the prsA gene (encoding a phosphoribosyl pyrophosphate synthetase) and the glpF gene (encoding a glycerol transporter), which are involved in an early key step in the biosynthetic pathway of nucleotides and amino acids (Trp and His) and the first step in glycerol utilization, respectively. As a result, the production of IGF-I-f could be increased from 1.8 +/- 0.13 (+/- standard deviation) to 4.3 +/- 0.24 g/liter. The volumetric productivity was also increased from 0.36 +/- 0.027 to 0.82 +/- 0.048 g/liter/h. These results demonstrate that transcriptome profiling can provide invaluable information in designing engineered strains showing enhanced performance. | - |
dc.description.sponsorship | This work was supported by the Basic Industrial Research Project of the Korean Ministry of Commerce, Industry and Energy (MOCIE) and by the Center for Ultramicrochemical Process Systems. Further support from LG Life Sciences Ltd., Samchully Pharmaceutical Co. Ltd., and BioLeaders Co. is also appreciated. Hardware for computational analysis was supported by the IBM Shared University Research Program. | en |
dc.language | English | - |
dc.language.iso | en_US | en |
dc.publisher | AMER SOC MICROBIOLOGY | - |
dc.title | Enhanced production of insulin-like growth factor I fusion protein in Escherichia coli by coexpression of the down-regulated genes identified by transcriptome profiling | - |
dc.type | Article | - |
dc.identifier.wosid | 000184672500053 | - |
dc.identifier.scopusid | 2-s2.0-0043031143 | - |
dc.type.rims | ART | - |
dc.citation.volume | 69 | - |
dc.citation.beginningpage | 4737 | - |
dc.citation.endingpage | 4742 | - |
dc.citation.publicationname | APPLIED AND ENVIRONMENTAL MICROBIOLOGY | - |
dc.embargo.liftdate | 9999-12-31 | - |
dc.embargo.terms | 9999-12-31 | - |
dc.contributor.localauthor | Lee, SangYup | - |
dc.contributor.nonIdAuthor | Choi, JH | - |
dc.contributor.nonIdAuthor | Seok Jae Lee | - |
dc.contributor.nonIdAuthor | Sang Jun Lee | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | CELL-DENSITY CULTURE | - |
dc.subject.keywordPlus | HIGH-LEVEL EXPRESSION | - |
dc.subject.keywordPlus | RECOMBINANT PROTEIN | - |
dc.subject.keywordPlus | PURIFICATION | - |
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