DC Field | Value | Language |
---|---|---|
dc.contributor.author | Jeong, Kijun | ko |
dc.contributor.author | Lee, SangYup | ko |
dc.date.accessioned | 2011-01-28T01:35:58Z | - |
dc.date.available | 2011-01-28T01:35:58Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2003-02 | - |
dc.identifier.citation | APPLIED AND ENVIRONMENTAL MICROBIOLOGY, v.69, no.2, pp.1295 - 1298 | - |
dc.identifier.issn | 0099-2240 | - |
dc.identifier.uri | http://hdl.handle.net/10203/21886 | - |
dc.description.abstract | During growth of high-cell-density cultures of Escherichia coli, overproduction of recombinant proteins often results in increased stress response, cell filamentation, and growth cessation. Filamentation of cells consequently lowers final achievable cell concentration and productivity of the target protein. Reported here is a methodology that should prove useful for the enhancement of cell growth and protein productivity by the suppression of cell filamentation. By the coexpression of the E. coli ftsA and ftsZ genes, which encode key proteins in cell division, growth of recombinant strains as well as production of human leptin and human insulin-like growth factor I was improved. Observation of cell morphology revealed that the coexpression of the ftsA and ftsZ genes successfully suppressed filamentation caused by the accumulation of recombinant proteins. | - |
dc.description.sponsorship | This work was supported by the Korean Ministry of Commerce, Industry, and Energy and by the National Research Laboratory program of the Korean Ministry of Science and Technology. Further support from LG Chem Investment Ltd., Samchully Pharmaceutical Co., and BioLeaders Co. is also appreciated. K.J.J. is a postdoctoral fellow supported by the Brain Korea 21 project. | en |
dc.language | English | - |
dc.language.iso | en_US | en |
dc.publisher | AMER SOC MICROBIOLOGY | - |
dc.title | Enhanced production of recombinant proteins in Escherichia coli by filamentation suppression | - |
dc.type | Article | - |
dc.identifier.wosid | 000180927100074 | - |
dc.identifier.scopusid | 2-s2.0-0037315884 | - |
dc.type.rims | ART | - |
dc.citation.volume | 69 | - |
dc.citation.issue | 2 | - |
dc.citation.beginningpage | 1295 | - |
dc.citation.endingpage | 1298 | - |
dc.citation.publicationname | APPLIED AND ENVIRONMENTAL MICROBIOLOGY | - |
dc.embargo.liftdate | 9999-12-31 | - |
dc.embargo.terms | 9999-12-31 | - |
dc.contributor.localauthor | Jeong, Kijun | - |
dc.contributor.localauthor | Lee, SangYup | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | CELL-DIVISION | - |
dc.subject.keywordPlus | FTSZ | - |
dc.subject.keywordPlus | PURIFICATION | - |
dc.subject.keywordPlus | RATIO | - |
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