High-level production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) by fed-batch culture of recombinant Escherichia coli

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dc.contributor.authorChoi, JIko
dc.contributor.authorLee, SangYupko
dc.date.accessioned2011-01-28T01:06:27Z-
dc.date.available2011-01-28T01:06:27Z-
dc.date.created2012-02-06-
dc.date.created2012-02-06-
dc.date.created2012-02-06-
dc.date.issued1999-10-
dc.identifier.citationAPPLIED AND ENVIRONMENTAL MICROBIOLOGY, v.65, no.10, pp.4363 - 4368-
dc.identifier.issn0099-2240-
dc.identifier.urihttp://hdl.handle.net/10203/21881-
dc.description.abstractFermentation strategies for production of high concentrations of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) [P(3HB-co-3HV)] with different 3-hydroxyvalerate (3HV) fractions by recombinant Escherichia coli harboring the Alcaligenes latus polyhydroxyalkanoate biosynthesis genes were developed. Fed-batch cultures of recombinant E. coli with the pa-stat feeding strategy facilitated production of high concentrations and high contents of P(3HB-co-3HV) in a chemically defined medium. When a feeding solution was added in order to increase the glucose and propionic acid concentrations to 20 g/liter and 20 mM, respectively, after each feeding, a cell dry weight of 120.3 g/liter and a relatively low P(3HB-co-3HV) content, 42.5 wt%, were obtained. Accumulation of a high residual concentration of propionic acid in the medium was the reason for the low P(3HB-co-3HV) content. An acetic acid induction strategy was used to stimulate the uptake and utilization of propionic acid. When a fed-batch culture and this strategy were used, we obtained a cell concentration, a P(3HB-co-3HV) concentration, a P(3HB-co-3HV) content, and a 3HV fraction of 141.9 g/liter, 88.1 g/liter, 62.1 wt%, and 15.3 mol%, respectively. When an improved nutrient feeding strategy, acetic acid induction, and oleic acid supplementation were used, we obtained a cell concentration, a P(3HB-co3HV) concentration, a P(3HB-co-3HV) content, and a 3HV fraction of 203.1 g/liter, 158.8 g/liter, 78.2 wt%, and 10.6 mol%, respectively; this resulted in a high level of productivity, 2.88 g of P(3HB-co3HV)/liter-h.-
dc.description.sponsorshipThis work was supported by the Ministry of Science and Technology and by LG Chemicals, Ltd.en
dc.languageEnglish-
dc.language.isoen_USen
dc.publisherAMER SOC MICROBIOLOGY-
dc.titleHigh-level production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) by fed-batch culture of recombinant Escherichia coli-
dc.typeArticle-
dc.identifier.wosid000082959100011-
dc.identifier.scopusid2-s2.0-0039109649-
dc.type.rimsART-
dc.citation.volume65-
dc.citation.issue10-
dc.citation.beginningpage4363-
dc.citation.endingpage4368-
dc.citation.publicationnameAPPLIED AND ENVIRONMENTAL MICROBIOLOGY-
dc.embargo.liftdate9999-12-31-
dc.embargo.terms9999-12-31-
dc.contributor.localauthorLee, SangYup-
dc.contributor.nonIdAuthorChoi, JI-
dc.type.journalArticleArticle-
dc.subject.keywordPlusBACTERIAL POLYHYDROXYALKANOATES-
dc.subject.keywordPlusPOLY-(3-HYDROXYBUTYRATE-CO-3-HYDROXYVALERATE)-
dc.subject.keywordPlusPOLY(3-HYDROXYBUTYRATE)-
dc.subject.keywordPlusACID-
dc.subject.keywordPlusBIOSYNTHESIS-
dc.subject.keywordPlusGROWTH-
dc.subject.keywordPlusGENES-
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