내부 필터 발효기에서 Halobacterium halobium의 배양에 의한 박테리오돕신의 생산

Abstract

Bacteriorhodopsin in the purple membrane(PM) of halobacteria has recently been attracting much attention to be used as a component of molecular electron device and optical computers. In order to increase the productivity of bacteriorhopsin in high cell density cultures of Halobacterium halobium R1, an internal membrane cell-retention bioreactor system was emplyed. As a result, the production of cell mass at OD660 of 12 and of Bacteriorhodopsin at 125-130mg/L were obtained using the internal membrane bioreactor system at a dilution rate of 0.066hr. The productivity achieved by the internal membrane system (0.7mg/L hr) was 3.5-fold higher than that obtained by the corresponding batch cultivations(0.2mg/L hr).