DC Field | Value | Language |
---|---|---|
dc.contributor.author | 최, 종일 | - |
dc.contributor.author | 박, 시재 | - |
dc.contributor.author | 이, 상엽 | - |
dc.contributor.author | Choi, Jong-Il | - |
dc.contributor.author | Park, Si Jae | - |
dc.contributor.author | Lee, Sang Yup | - |
dc.date.accessioned | 2011-01-27T02:33:17Z | - |
dc.date.available | 2011-01-27T02:33:17Z | - |
dc.date.issued | 2004 | - |
dc.identifier.citation | Korean Journal of Biotechnology and Bioengineering, Vol.19, No.4, pp.331-334 | en |
dc.identifier.uri | http://hdl.handle.net/10203/21844 | - |
dc.description.abstract | In vivo characterization of FadB homologous enzymes including PaaG, YdbU and YgfG for medium-chain-length(MCL) Polyhydroxyalkanoates(PHA) biosynthesis was carried out in fadB mutant Escherichia coli. Previously, it was reported that amplificaion of FadB homologous enzyme such as PaaG and YdbU in fadB mutnat E.coli resulted in enhanced biosynthesis of MCL-PHA by greater than two fold compared with control strain. In this study, we constructed paaG fadB double mutant E.coli WB114 and ydbU fadB double mutant E.coli WB115 to investigate the roles of PaaG and YdbU in biosynthesis of MCL-PHA. Inactivation of paaG and ydbU genes in fadB mutant E.coli harboring Pseudomonas sp.61-3 phaC2 gene reduced the MCL-PHA production to 0.16 and 0.16 PHA g/L, respectively from 2 g/L of sodium decanoate, which are much lower than 0.43 PHA g/L obtained with fadB mutant E.coli WB101 harboring the phaC2 gene. Also, we identified new FadB homologous enzyme YgfG, and examined its roles by overexpression of ygfG and construction of ygfG fadB double mutant E.coli WB113. | en |
dc.description.sponsorship | 이 연구는 과학기술부 국가지정연구실 (National Research Laboratory) 사업과 BK21 program의 연구비 지원에 의하여 수행되었으며, 이에 감사드립니다. | en |
dc.language.iso | ko | en |
dc.publisher | 한국생물공학회 | en |
dc.subject | paaG | en |
dc.subject | ydbU | en |
dc.subject | ygfG | en |
dc.subject | fadB | en |
dc.subject | recombinant E. coli | en |
dc.subject | PHA | en |
dc.title | 재조합 대장균에서 fadB 유사효소의 Polyhydroxyalkanoates 합성에 미치는 역할의 규명 | en |
dc.title.alternative | In Vivo Analysis of fadB Homologous Enzymes Involved in Biosynthesis of Polyhydroxyalkanoates in Recombinant Escherichia coli | en |
dc.type | Article | en |
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