DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Che Lin | ko |
dc.contributor.author | Bang, You Lim | ko |
dc.contributor.author | Kim, Young Sik | ko |
dc.contributor.author | Jang, Ju Woong | ko |
dc.contributor.author | Lee, Gyun Min | ko |
dc.date.accessioned | 2016-10-04T08:57:49Z | - |
dc.date.available | 2016-10-04T08:57:49Z | - |
dc.date.created | 2016-09-21 | - |
dc.date.created | 2016-09-21 | - |
dc.date.issued | 2016-08 | - |
dc.identifier.citation | PROCESS BIOCHEMISTRY, v.51, no.8, pp.1078 - 1084 | - |
dc.identifier.issn | 1359-5113 | - |
dc.identifier.uri | http://hdl.handle.net/10203/213167 | - |
dc.description.abstract | The effects of culture temperature and pH on cell growth and recombinant human bone morphogenetic protein-4 (rhBMP-4) production were investigated to maximize rhBMP-4 production from Chinese hamster ovary cells. Cells were cultivated in a bioreactor at different culture pH levels (6.7, 6.9, 7.2, and 7.5) and temperatures (37 degrees C and 33 degrees C). The highest rhBMP-4 concentration (4.6 +/- 0.5 mu g/mL) was obtained at pH 7.2 and 37 degrees C. Regardless of culture temperature and pH, significant rhBMP-4 degradation via increased proteolytic activity was observed in the decline growth phase. Protease inhibitors such as EDTA and leupeptin were toxic and thus did not enhance rhBMP-4 production. High viability was achieved in repeated batch cultures with frequent medium exchanges. These cultures showed enhanced rhBMP-4 productivity, demonstrating the potential of repeated batch cultures as a means for improved rhBMP-4 production. (C) 2016 Elsevier Ltd. All rights reserved | - |
dc.language | English | - |
dc.publisher | ELSEVIER SCI LTD | - |
dc.subject | CHO-CELLS | - |
dc.subject | PROTEASE INHIBITORS | - |
dc.subject | TEMPERATURE | - |
dc.subject | PRODUCTIVITY | - |
dc.subject | ERYTHROPOIETIN | - |
dc.subject | HETEROGENEITY | - |
dc.subject | EXPRESSION | - |
dc.subject | LEVEL | - |
dc.subject | PH | - |
dc.subject | OVEREXPRESSION | - |
dc.title | Alleviation of proteolytic degradation of recombinant human bone morphogenetic protein-4 by repeated batch culture of Chinese hamster ovary cells | - |
dc.type | Article | - |
dc.identifier.wosid | 000380972500017 | - |
dc.identifier.scopusid | 2-s2.0-84975457983 | - |
dc.type.rims | ART | - |
dc.citation.volume | 51 | - |
dc.citation.issue | 8 | - |
dc.citation.beginningpage | 1078 | - |
dc.citation.endingpage | 1084 | - |
dc.citation.publicationname | PROCESS BIOCHEMISTRY | - |
dc.identifier.doi | 10.1016/j.procbio.2016.05.007 | - |
dc.contributor.localauthor | Lee, Gyun Min | - |
dc.contributor.nonIdAuthor | Bang, You Lim | - |
dc.contributor.nonIdAuthor | Kim, Young Sik | - |
dc.contributor.nonIdAuthor | Jang, Ju Woong | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | BMP-4 | - |
dc.subject.keywordAuthor | CHO cells | - |
dc.subject.keywordAuthor | Culture temperature | - |
dc.subject.keywordAuthor | Culture pH | - |
dc.subject.keywordAuthor | Proteolytic degradation | - |
dc.subject.keywordAuthor | Repeated batch cultures | - |
dc.subject.keywordPlus | CHO-CELLS | - |
dc.subject.keywordPlus | PROTEASE INHIBITORS | - |
dc.subject.keywordPlus | TEMPERATURE | - |
dc.subject.keywordPlus | PRODUCTIVITY | - |
dc.subject.keywordPlus | ERYTHROPOIETIN | - |
dc.subject.keywordPlus | HETEROGENEITY | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | LEVEL | - |
dc.subject.keywordPlus | PH | - |
dc.subject.keywordPlus | OVEREXPRESSION | - |
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