DC Field | Value | Language |
---|---|---|
dc.contributor.author | Xu, Z. | ko |
dc.contributor.author | Lee, SangYup | ko |
dc.contributor.author | Yu, Z. | ko |
dc.date.accessioned | 2010-12-27T09:18:10Z | - |
dc.date.available | 2010-12-27T09:18:10Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 1999-12 | - |
dc.identifier.citation | BIOTECHNOLOGY LETTERS, v.21, no.12, pp.1091 - 1094 | - |
dc.identifier.issn | 0141-5492 | - |
dc.identifier.uri | http://hdl.handle.net/10203/21248 | - |
dc.description.abstract | Using the Escherichia coli OmpC protein as an anchoring motif, four different poly-His units (1, 2, 3 and 6 copies of 6-His) were displayed on the seventh loop of the OmpC. Recombinant E. coli strains displaying 1, 3 or 6 copies of poly-His became much more sensitive to SDS (0.1%, w/v) and EDTA (2 mM) compared with control strains. However, recombinant E. coli cells displaying 2 copies of poly-His were resistant to SDS and EDTA; greater than 70% and 90% of cells maintained cell integrity after 60 min treatment with SDS and EDTA, respectively, suggesting its usefulness as a whole cell biosorbent. | - |
dc.description.sponsorship | This work was supported by the First Young Scientist’s Award to SYL by the President of Korea and by the Korea Academy of Science and Technology. Z. Xu is a visiting Ph.D. student from Huazhong Agricultural University, China, and has been partially supported by the BioProcess Engineering Research Center. | en |
dc.language | English | - |
dc.language.iso | en_US | en |
dc.publisher | KLUWER ACADEMIC PUBL | - |
dc.title | Physiological characteristics of recombinant Escherichia coli cells displaying poly-His peptides | - |
dc.type | Article | - |
dc.identifier.wosid | 000084811200012 | - |
dc.identifier.scopusid | 2-s2.0-0033373609 | - |
dc.type.rims | ART | - |
dc.citation.volume | 21 | - |
dc.citation.issue | 12 | - |
dc.citation.beginningpage | 1091 | - |
dc.citation.endingpage | 1094 | - |
dc.citation.publicationname | BIOTECHNOLOGY LETTERS | - |
dc.identifier.doi | 10.1023/A:1005618025127 | - |
dc.embargo.liftdate | 9999-12-31 | - |
dc.embargo.terms | 9999-12-31 | - |
dc.contributor.localauthor | Lee, SangYup | - |
dc.contributor.nonIdAuthor | Xu, Z. | - |
dc.contributor.nonIdAuthor | Yu, Z. | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | cell surface display | - |
dc.subject.keywordAuthor | Escherichia coli | - |
dc.subject.keywordAuthor | poly-His | - |
dc.subject.keywordPlus | SURFACE DISPLAY | - |
dc.subject.keywordPlus | STAPHYLOCOCCUS-XYLOSUS | - |
dc.subject.keywordPlus | YEAST | - |
dc.subject.keywordPlus | METALLOADSORPTION | - |
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