DC Field | Value | Language |
---|---|---|
dc.contributor.author | Park, SJ | ko |
dc.contributor.author | Georgiou, G | ko |
dc.contributor.author | Lee, SangYup | ko |
dc.date.accessioned | 2010-12-24T04:58:17Z | - |
dc.date.available | 2010-12-24T04:58:17Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 1999-03 | - |
dc.identifier.citation | BIOTECHNOLOGY PROGRESS, v.15, no.2, pp.164 - 167 | - |
dc.identifier.issn | 8756-7938 | - |
dc.identifier.uri | http://hdl.handle.net/10203/21225 | - |
dc.description.abstract | Several protease negative mutant strains including HM114, HM126, and HM130 as well as their parent strain KS272 were compared for their growth and secretory production of a model fusion protein, protein A-beta-lactamase. HM114, a strain deficient in two cell envelope proteases, grew slightly faster and produced more fusion protein than the other strains deficient in more proteases. HM114 was grown to a cell dry weight of 47.86 g/L in 29 h using pH-stat, fed-batch cultivation. The beta-lactamase activity was 11.25 x 10(4) U/L, which was 30% higher than that obtained with its parent strain KS272. Up to 96% of protein A-beta-lactamase fusion protein could be recovered by a simple cold osmotic shock method. The specific beta-lactamase activity obtained with HM114 after fractionation was 4.5 times higher than that obtained with KS272. | - |
dc.description.sponsorship | This study is supported by the academic research fund from the Ministry of Education, Korea. | en |
dc.language | English | - |
dc.language.iso | en_US | en |
dc.publisher | AMER CHEMICAL SOC | - |
dc.title | Secretory production of recombinant protein by a high cell density culture of a protease negative mutant Escherichia coli strain | - |
dc.type | Article | - |
dc.identifier.wosid | 000079641600003 | - |
dc.identifier.scopusid | 2-s2.0-0345491422 | - |
dc.type.rims | ART | - |
dc.citation.volume | 15 | - |
dc.citation.issue | 2 | - |
dc.citation.beginningpage | 164 | - |
dc.citation.endingpage | 167 | - |
dc.citation.publicationname | BIOTECHNOLOGY PROGRESS | - |
dc.embargo.liftdate | 9999-12-31 | - |
dc.embargo.terms | 9999-12-31 | - |
dc.contributor.localauthor | Lee, SangYup | - |
dc.contributor.nonIdAuthor | Park, SJ | - |
dc.contributor.nonIdAuthor | Georgiou, G | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | CONSTRUCTION | - |
dc.subject.keywordPlus | PROTEOLYSIS | - |
dc.subject.keywordPlus | DEFICIENT | - |
dc.subject.keywordPlus | INVIVO | - |
dc.subject.keywordPlus | GENE | - |
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