DC Field | Value | Language |
---|---|---|
dc.contributor.author | Nguyen, Mai Khanh | ko |
dc.contributor.author | Kim, Cha Yeon | ko |
dc.contributor.author | Kim, Jin Man | ko |
dc.contributor.author | Park, Byung Ouk | ko |
dc.contributor.author | Lee, Sangkyu | ko |
dc.contributor.author | Park, Hyerim | ko |
dc.contributor.author | Heo, Won Do | ko |
dc.date.accessioned | 2016-07-06T04:18:47Z | - |
dc.date.available | 2016-07-06T04:18:47Z | - |
dc.date.created | 2016-06-08 | - |
dc.date.created | 2016-06-08 | - |
dc.date.created | 2016-06-08 | - |
dc.date.issued | 2016-06 | - |
dc.identifier.citation | NATURE CHEMICAL BIOLOGY, v.12, no.6, pp.431 - U86 | - |
dc.identifier.issn | 1552-4450 | - |
dc.identifier.uri | http://hdl.handle.net/10203/209475 | - |
dc.description.abstract | Intracellular membrane trafficking, which is involved in diverse cellular processes, is dynamic and difficult to study in a spatiotemporal manner. Here we report an optogenetic strategy, termed light-activated reversible inhibition by assembled trap of intracellular membranes (IM-LARIAT), that uses various Rab GTPases combined with blue-light-induced hetero-interaction between cryptochrome 2 and CIB1. In this system, illumination induces a rapid and reversible intracellular membrane aggregation that disrupts the dynamics and functions of the targeted membrane. We applied IM-LARIAT to specifically perturb several Rab-mediated trafficking processes, including receptor transport, protein sorting and secretion, and signaling initiated from endosomes. We finally used this tool to reveal different functions of local Rab5-mediated and Rab11-mediated membrane trafficking in growth cones and soma of young hippocampal neurons. Our results show that IM-LARIAT is a versatile tool that can be used to dissect spatiotemporal functions of intracellular membranes in diverse systems | - |
dc.language | English | - |
dc.publisher | NATURE PUBLISHING GROUP | - |
dc.title | Optogenetic oligomerization of Rab GTPases regulates intracellular membrane trafficking | - |
dc.type | Article | - |
dc.identifier.wosid | 000376160600013 | - |
dc.identifier.scopusid | 2-s2.0-84970027518 | - |
dc.type.rims | ART | - |
dc.citation.volume | 12 | - |
dc.citation.issue | 6 | - |
dc.citation.beginningpage | 431 | - |
dc.citation.endingpage | U86 | - |
dc.citation.publicationname | NATURE CHEMICAL BIOLOGY | - |
dc.identifier.doi | 10.1038/NCHEMBIO.2064 | - |
dc.contributor.localauthor | Heo, Won Do | - |
dc.contributor.nonIdAuthor | Park, Byung Ouk | - |
dc.contributor.nonIdAuthor | Lee, Sangkyu | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | GROWTH-FACTOR | - |
dc.subject.keywordPlus | ENDOCYTIC TRAFFICKING | - |
dc.subject.keywordPlus | TRANSFERRIN RECEPTOR | - |
dc.subject.keywordPlus | LIVING CELLS | - |
dc.subject.keywordPlus | AXON GROWTH | - |
dc.subject.keywordPlus | PC12 CELLS | - |
dc.subject.keywordPlus | BLUE-LIGHT | - |
dc.subject.keywordPlus | ACTIVATION | - |
dc.subject.keywordPlus | TRANSPORT | - |
dc.subject.keywordPlus | ENDOSOME | - |
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