DC Field | Value | Language |
---|---|---|
dc.contributor.author | Che, Hui-Lian | ko |
dc.contributor.author | Bae, In-Ho | ko |
dc.contributor.author | Lim, Kyung Seob | ko |
dc.contributor.author | Song, In Taek | ko |
dc.contributor.author | Lee, Haeshin | ko |
dc.contributor.author | Lee, Duhwan | ko |
dc.contributor.author | Kim, Won Jong | ko |
dc.contributor.author | Jeong, Myung-Ho | ko |
dc.contributor.author | Park, In-Kyu | ko |
dc.contributor.author | Ahn, Youngkeun | ko |
dc.date.accessioned | 2016-07-06T04:18:23Z | - |
dc.date.available | 2016-07-06T04:18:23Z | - |
dc.date.created | 2016-06-13 | - |
dc.date.created | 2016-06-13 | - |
dc.date.issued | 2016-06 | - |
dc.identifier.citation | JOURNAL OF BIOMEDICAL NANOTECHNOLOGY, v.12, no.6, pp.1211 - 1222 | - |
dc.identifier.issn | 1550-7033 | - |
dc.identifier.uri | http://hdl.handle.net/10203/209471 | - |
dc.description.abstract | For effective treatment of restenosis, therapeutic genes are delivered locally from a coated stent at the site of injury, leading to inhibition of smooth muscle proliferation and neo-intimal hyperplasia while promoting re-endothelialization. In a previous study, we delivered Akt1 siRNA nanoparticles (ASNs) from a hyaluronic acid (HA)-coated stent surface to specifically suppress the pro-proliferative Akt1 protein in smooth muscle cells (SMCs). In the present study, therapeutic efficacy was investigated in a rabbit restenosis model after percutaneous implantation of an ASN-immobilized stent in a rabbit iliac artery. Quantitative and qualitative analyses of in-stent restenosis were investigated in an in vivo animal model by micro-CT imaging and SEM observation, respectively. Proliferation status and neo-intima formation of the vascular tissues located near ASN-immobilized stents were analyzed by immunohistochemical staining using anti-Akt1 and anti-Ki67 antibodies and histological analyses, such as hematoxylin and eosin staining and Verhoeff's elastic stain. Re-endothelialization after implantation of an ASN-immobilized stent was also analyzed via immunohistochemistry using an anti-CD31 antibody. To elucidate the molecular mechanism related to reducing SMC proliferation and subsequent inhibition of in-stent restenosis in vivo, protein and mRNA expression of Akt1 and downstream signaling proteins were analyzed after isolating SMC-rich samples from the treated vasculature. The implanted Akt1 siRNA-eluting stent efficiently mitigated in-stent restenosis without any side effects and can be considered a successful substitute to current drug-eluting stents | - |
dc.language | English | - |
dc.publisher | AMER SCIENTIFIC PUBLISHERS | - |
dc.subject | GENE-THERAPY | - |
dc.subject | ARTERY STENTS | - |
dc.subject | DELIVERY | - |
dc.subject | SUPPLEMENTATION | - |
dc.subject | EFFICACY | - |
dc.subject | SAFETY | - |
dc.subject | CELLS | - |
dc.subject | MODEL | - |
dc.title | Therapeutic Effect of Akt1 siRNA Nanoparticle Eluting Coronary Stent on Suppression of Post-Angioplasty Restenosis | - |
dc.type | Article | - |
dc.identifier.wosid | 000375968500007 | - |
dc.identifier.scopusid | 2-s2.0-84964720694 | - |
dc.type.rims | ART | - |
dc.citation.volume | 12 | - |
dc.citation.issue | 6 | - |
dc.citation.beginningpage | 1211 | - |
dc.citation.endingpage | 1222 | - |
dc.citation.publicationname | JOURNAL OF BIOMEDICAL NANOTECHNOLOGY | - |
dc.identifier.doi | 10.1166/jbn.2016.2255 | - |
dc.contributor.localauthor | Lee, Haeshin | - |
dc.contributor.nonIdAuthor | Che, Hui-Lian | - |
dc.contributor.nonIdAuthor | Bae, In-Ho | - |
dc.contributor.nonIdAuthor | Lim, Kyung Seob | - |
dc.contributor.nonIdAuthor | Lee, Duhwan | - |
dc.contributor.nonIdAuthor | Kim, Won Jong | - |
dc.contributor.nonIdAuthor | Jeong, Myung-Ho | - |
dc.contributor.nonIdAuthor | Park, In-Kyu | - |
dc.contributor.nonIdAuthor | Ahn, Youngkeun | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | Restenosis | - |
dc.subject.keywordAuthor | Akt1 siRNA | - |
dc.subject.keywordAuthor | Hyaluronic Acid | - |
dc.subject.keywordAuthor | Coronary Stent | - |
dc.subject.keywordAuthor | Smooth Muscle Cells | - |
dc.subject.keywordAuthor | Gene Eluting Stents | - |
dc.subject.keywordAuthor | Micro-CT Imaging | - |
dc.subject.keywordPlus | GENE-THERAPY | - |
dc.subject.keywordPlus | ARTERY STENTS | - |
dc.subject.keywordPlus | DELIVERY | - |
dc.subject.keywordPlus | SUPPLEMENTATION | - |
dc.subject.keywordPlus | EFFICACY | - |
dc.subject.keywordPlus | SAFETY | - |
dc.subject.keywordPlus | CELLS | - |
dc.subject.keywordPlus | MODEL | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.