DC Field | Value | Language |
---|---|---|
dc.contributor.author | Hyun, Sang Hwa | ko |
dc.contributor.author | Park, Dae Keun | ko |
dc.contributor.author | Kang, Aeyeon | ko |
dc.contributor.author | Kim, Soohyun | ko |
dc.contributor.author | Kim, Daehee | ko |
dc.contributor.author | Shin, Yu Mi | ko |
dc.contributor.author | Song, Ji-Joon | ko |
dc.contributor.author | Yun, Wan Soo | ko |
dc.date.accessioned | 2016-07-05T08:22:54Z | - |
dc.date.available | 2016-07-05T08:22:54Z | - |
dc.date.created | 2016-05-31 | - |
dc.date.created | 2016-05-31 | - |
dc.date.issued | 2016-02 | - |
dc.identifier.citation | APPLIED PHYSICS LETTERS, v.108, no.9 | - |
dc.identifier.issn | 0003-6951 | - |
dc.identifier.uri | http://hdl.handle.net/10203/209365 | - |
dc.description.abstract | We report a simple label-free electrochemical method of detecting low concentrations of botulinum neurotoxin type E light chain (BoNT/E LC) based on its peptide cleavage activity. Dual-mode cyclic voltammetry was employed to observe changes in the redox signal of ferri-/ferro-cyanide on interdigitated microelectrodes, whose surfaces were covered by peptides designed from synaptosomal-associated protein 25 to be cleaved by BoNT/E LC. With the introduction of BoNT/E LC, the redox signal showed a time-dependent increase due to cleavage of the immobilized peptide molecules. In addition to the increased redox signal intensity, its time-dependence can be considered as a strong evidence of BoNT/E sensing, since the time-dependent increase can only result from the enzymatic activity of BoNT/E LC. Using this method, BoNT/E LC, at concentrations as low as 5 pg/ml, was readily measurable with only an hour of incubation. (C) 2016 AIP Publishing LLC | - |
dc.language | English | - |
dc.publisher | AMER INST PHYSICS | - |
dc.subject | GOLD NANOPARTICLES | - |
dc.subject | TOXIN | - |
dc.subject | IMMUNOSENSOR | - |
dc.subject | DIAGNOSIS | - |
dc.subject | SURFACES | - |
dc.subject | ASSAY | - |
dc.title | Label-free electrochemical detection of botulinum neurotoxin type E based on its enzymatic activity using interdigitated electrodes | - |
dc.type | Article | - |
dc.identifier.wosid | 000375329200041 | - |
dc.identifier.scopusid | 2-s2.0-84959520164 | - |
dc.type.rims | ART | - |
dc.citation.volume | 108 | - |
dc.citation.issue | 9 | - |
dc.citation.publicationname | APPLIED PHYSICS LETTERS | - |
dc.identifier.doi | 10.1063/1.4942800 | - |
dc.contributor.localauthor | Song, Ji-Joon | - |
dc.contributor.nonIdAuthor | Hyun, Sang Hwa | - |
dc.contributor.nonIdAuthor | Park, Dae Keun | - |
dc.contributor.nonIdAuthor | Kang, Aeyeon | - |
dc.contributor.nonIdAuthor | Kim, Soohyun | - |
dc.contributor.nonIdAuthor | Kim, Daehee | - |
dc.contributor.nonIdAuthor | Shin, Yu Mi | - |
dc.contributor.nonIdAuthor | Yun, Wan Soo | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | GOLD NANOPARTICLES | - |
dc.subject.keywordPlus | TOXIN | - |
dc.subject.keywordPlus | IMMUNOSENSOR | - |
dc.subject.keywordPlus | DIAGNOSIS | - |
dc.subject.keywordPlus | SURFACES | - |
dc.subject.keywordPlus | ASSAY | - |
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