DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Min Jeong | ko |
dc.contributor.author | Yim, Sung Sun | ko |
dc.contributor.author | Choi, Jae Woong | ko |
dc.contributor.author | Jeong, Ki Jun | ko |
dc.date.accessioned | 2016-07-05T08:17:16Z | - |
dc.date.available | 2016-07-05T08:17:16Z | - |
dc.date.created | 2016-05-31 | - |
dc.date.created | 2016-05-31 | - |
dc.date.created | 2016-05-31 | - |
dc.date.issued | 2016-05 | - |
dc.identifier.citation | APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, v.100, no.10, pp.4473 - 4483 | - |
dc.identifier.issn | 0175-7598 | - |
dc.identifier.uri | http://hdl.handle.net/10203/209301 | - |
dc.description.abstract | Corynebacterium glutamicum is a non-pathogenic, non-sporulating Gram-positive soil bacterium that has been used for the industrial production of various proteins and chemicals. To achieve enhanced and economical production of target molecules, the development of strong auto-inducible promoters is desired, which can be activated without expensive inducers and has significant advantages for industrial-scale use. Here, we developed a stationary-phase gene expression system by engineering a sigma factor B (SigB)-dependent promoter that can be activated during the transition phase between exponential and stationary growth phases in C. glutamicum. First, the inducibilities of three well-known SigB-dependent promoters were examined using super-folder green fluorescent protein as a reporter protein, and we found that promoter of cg3141 (P (cg3141) ) exhibited the highest inducibility. Next, a synthetic promoter library was constructed by randomizing the flanking and space regions of P (cg3141) , and the stationary-phase promoters exhibiting high strengths were isolated via FACS-based high-throughput screening. The isolated synthetic promoter (P4-N14) showed a 3.5-fold inducibility and up to 20-fold higher strength compared to those of the original cg3141 promoter. Finally, the use of the isolated P4-N14 for fed-batch cultivation was verified with the production of glutathione S-transferase as a model protein in a lab-scale (5-L) bioreactor | - |
dc.language | English | - |
dc.publisher | SPRINGER | - |
dc.title | Development of a potential stationary-phase specific gene expression system by engineering of SigB-dependent cg3141 promoter in Corynebacterium glutamicum | - |
dc.type | Article | - |
dc.identifier.wosid | 000375057700019 | - |
dc.identifier.scopusid | 2-s2.0-84954481272 | - |
dc.type.rims | ART | - |
dc.citation.volume | 100 | - |
dc.citation.issue | 10 | - |
dc.citation.beginningpage | 4473 | - |
dc.citation.endingpage | 4483 | - |
dc.citation.publicationname | APPLIED MICROBIOLOGY AND BIOTECHNOLOGY | - |
dc.identifier.doi | 10.1007/s00253-016-7297-y | - |
dc.contributor.localauthor | Yim, Sung Sun | - |
dc.contributor.localauthor | Jeong, Ki Jun | - |
dc.contributor.nonIdAuthor | Kim, Min Jeong | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | Corynebacterium glutamicum | - |
dc.subject.keywordAuthor | Stationary-phase promoter | - |
dc.subject.keywordAuthor | cg3141 | - |
dc.subject.keywordAuthor | Sigma factor B | - |
dc.subject.keywordAuthor | FACS | - |
dc.subject.keywordPlus | ESCHERICHIA-COLI | - |
dc.subject.keywordPlus | GLUTAMATE-DECARBOXYLASE | - |
dc.subject.keywordPlus | RECOMBINANT PROTEINS | - |
dc.subject.keywordPlus | STRESS | - |
dc.subject.keywordPlus | OPERON | - |
dc.subject.keywordPlus | METABOLISM | - |
dc.subject.keywordPlus | REGULATOR | - |
dc.subject.keywordPlus | LIBRARIES | - |
dc.subject.keywordPlus | NARKGHJI | - |
dc.subject.keywordPlus | SEQUENCE | - |
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