DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kang, Mingchao | ko |
dc.contributor.author | Light, Kenneth | ko |
dc.contributor.author | Ai, Hui-wang | ko |
dc.contributor.author | Shen, Weijun | ko |
dc.contributor.author | Kim, Chan Hyuk | ko |
dc.contributor.author | Chen, Peng R. | ko |
dc.contributor.author | Lee, Hyun Soo | ko |
dc.contributor.author | Solomon, Edward I. | ko |
dc.contributor.author | Schultz, Peter G. | ko |
dc.date.accessioned | 2016-07-04T06:02:22Z | - |
dc.date.available | 2016-07-04T06:02:22Z | - |
dc.date.created | 2016-05-13 | - |
dc.date.created | 2016-05-13 | - |
dc.date.created | 2016-05-13 | - |
dc.date.issued | 2014-04 | - |
dc.identifier.citation | CHEMBIOCHEM, v.15, no.6, pp.822 - 825 | - |
dc.identifier.issn | 1439-4227 | - |
dc.identifier.uri | http://hdl.handle.net/10203/209130 | - |
dc.description.abstract | We report the engineering of zinc-finger-like motifs containing the unnatural amino acid (2,2-bipyridin-5-yl)alanine (Bpy-Ala). A phage-display library was constructed in which five residues in the N-terminal finger of zif268 were randomized to include both canonical amino acids and Bpy-Ala. Panning of this library against a nine-base-pair DNA binding site identified several Bpy-Ala-containing functional Zif268 mutants. These mutants bind the Zif268 recognition site with affinities comparable to that of the wild-type protein. Further characterization indicated that the mutant fingers bind low-spin Fe-II rather than Zn-II. This work demonstrates that an expanded genetic code can lead to new metal ion binding motifs that can serve as structural, catalytic, or regulatory elements in proteins. | - |
dc.language | English | - |
dc.publisher | WILEY-V C H VERLAG GMBH | - |
dc.subject | FINGER DNA RECOGNITION | - |
dc.subject | IRON(III) HETEROLIGAND COMPLEXES | - |
dc.subject | CRYSTAL-STRUCTURE DETERMINATION | - |
dc.subject | MIXED-LIGAND COMPLEXES | - |
dc.subject | ESCHERICHIA-COLI | - |
dc.subject | GENETIC-CODE | - |
dc.subject | PHAGE DISPLAY | - |
dc.subject | ZINC FINGERS | - |
dc.subject | DESIGN | - |
dc.subject | CHLORIDE | - |
dc.title | Evolution of Iron(II)-Finger Peptides by Using a Bipyridyl Amino Acid | - |
dc.type | Article | - |
dc.identifier.wosid | 000333966100008 | - |
dc.identifier.scopusid | 2-s2.0-84898546801 | - |
dc.type.rims | ART | - |
dc.citation.volume | 15 | - |
dc.citation.issue | 6 | - |
dc.citation.beginningpage | 822 | - |
dc.citation.endingpage | 825 | - |
dc.citation.publicationname | CHEMBIOCHEM | - |
dc.identifier.doi | 10.1002/cbic.201300727 | - |
dc.contributor.localauthor | Kim, Chan Hyuk | - |
dc.contributor.nonIdAuthor | Kang, Mingchao | - |
dc.contributor.nonIdAuthor | Light, Kenneth | - |
dc.contributor.nonIdAuthor | Ai, Hui-wang | - |
dc.contributor.nonIdAuthor | Shen, Weijun | - |
dc.contributor.nonIdAuthor | Chen, Peng R. | - |
dc.contributor.nonIdAuthor | Lee, Hyun Soo | - |
dc.contributor.nonIdAuthor | Solomon, Edward I. | - |
dc.contributor.nonIdAuthor | Schultz, Peter G. | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | amino acids | - |
dc.subject.keywordAuthor | bipyridyl complexes | - |
dc.subject.keywordAuthor | iron | - |
dc.subject.keywordAuthor | phage display | - |
dc.subject.keywordAuthor | zinc finger engineering | - |
dc.subject.keywordPlus | FINGER DNA RECOGNITION | - |
dc.subject.keywordPlus | IRON(III) HETEROLIGAND COMPLEXES | - |
dc.subject.keywordPlus | CRYSTAL-STRUCTURE DETERMINATION | - |
dc.subject.keywordPlus | MIXED-LIGAND COMPLEXES | - |
dc.subject.keywordPlus | ESCHERICHIA-COLI | - |
dc.subject.keywordPlus | GENETIC-CODE | - |
dc.subject.keywordPlus | PHAGE DISPLAY | - |
dc.subject.keywordPlus | ZINC FINGERS | - |
dc.subject.keywordPlus | DESIGN | - |
dc.subject.keywordPlus | CHLORIDE | - |
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