DC Field | Value | Language |
---|---|---|
dc.contributor.author | Han, Dongsik | ko |
dc.contributor.author | Park, Je-Kyun | ko |
dc.date.accessioned | 2016-07-04T03:11:59Z | - |
dc.date.available | 2016-07-04T03:11:59Z | - |
dc.date.created | 2016-04-27 | - |
dc.date.created | 2016-04-27 | - |
dc.date.issued | 2016-04 | - |
dc.identifier.citation | LAB ON A CHIP, v.16, no.7, pp.1189 - 1196 | - |
dc.identifier.issn | 1473-0197 | - |
dc.identifier.uri | http://hdl.handle.net/10203/209029 | - |
dc.description.abstract | We report a novel optoelectrofluidic immunoreaction system based on electroosmotic flow for enhancing antibody-analyte binding efficiency on a surface-based sensing system. Two conventional indium tin oxide glass slides are assembled to provide a reaction chamber for a tiny volume of sample droplet (similar to 5 mu L), in which the top layer is employed as an antibody-immobilized substrate and the bottom layer acts as a photoconductive layer of an optoelectrofluidic device. Under the application of an AC voltage, an illuminated light pattern on the photoconductive layer causes strong counter-rotating vortices to transport analytes from the bulk solution to the vicinity of the assay spot on the glass substrate. This configuration overcomes the slow immunoreaction problem of a diffusion-based sensing system, resulting in the enhancement of binding efficiency via an optoelectrofluidic method. Furthermore, we investigate the effect of optically-induced dynamic AC electroosmotic flow on optoelectrofluidic enhancement for surface-based immunoreaction with a mathematical simulation study and real experiments using immunoglobulin G (IgG) and anti-IgG. As a result, dynamic light patterns provided better immunoreaction efficiency than static light patterns due to effective mass transport of the target analyte, resulting in an achievement of 2.18-fold enhancement under a growing circular light pattern compared to the passive mode | - |
dc.language | English | - |
dc.publisher | ROYAL SOC CHEMISTRY | - |
dc.title | Optoelectrofluidic enhanced immunoreaction based on optically-induced dynamic AC electroosmosis | - |
dc.type | Article | - |
dc.identifier.wosid | 000373057400010 | - |
dc.identifier.scopusid | 2-s2.0-84962094735 | - |
dc.type.rims | ART | - |
dc.citation.volume | 16 | - |
dc.citation.issue | 7 | - |
dc.citation.beginningpage | 1189 | - |
dc.citation.endingpage | 1196 | - |
dc.citation.publicationname | LAB ON A CHIP | - |
dc.identifier.doi | 10.1039/c6lc00110f | - |
dc.contributor.localauthor | Park, Je-Kyun | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | MICROARRAY | - |
dc.subject.keywordPlus | MICROPARTICLES | - |
dc.subject.keywordPlus | IMMUNOASSAYS | - |
dc.subject.keywordPlus | MANIPULATION | - |
dc.subject.keywordPlus | DIFFUSION | - |
dc.subject.keywordPlus | ANTIBODY | - |
dc.subject.keywordPlus | PLATFORM | - |
dc.subject.keywordPlus | CELLS | - |
dc.subject.keywordPlus | FLUID | - |
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