DC Field | Value | Language |
---|---|---|
dc.contributor.author | Jiang, H | ko |
dc.contributor.author | Shang, L | ko |
dc.contributor.author | Yoon, SH | ko |
dc.contributor.author | Lee, SangYup | ko |
dc.contributor.author | Yu, Z | ko |
dc.date.accessioned | 2010-12-09T02:20:50Z | - |
dc.date.available | 2010-12-09T02:20:50Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2006-08 | - |
dc.identifier.citation | BIOTECHNOLOGY LETTERS, v.28, pp.1241 - 1246 | - |
dc.identifier.issn | 0141-5492 | - |
dc.identifier.uri | http://hdl.handle.net/10203/20872 | - |
dc.description.abstract | Metabolically-engineered Escherichia coli strains were developed by cloning poly-gamma-glutamic acid (gamma-PGA) biosynthesis genes, consisting of pgsB, pgsC and pgsA, from Bacillus subtilis The metabolic and regulatory pathways of gamma-PGA biosynthesis in E. coli were analyzed by DNA microarray. The inducible trc promoter and a constitutive promoter (P-HCE) derived from the D-amino acid aminotransferase (D-AAT) gene of Geobacillus toebii were employed. The constitutive HCE promoter was more efficient than inducible trc promoter for the expression of gamma-PGA biosynthesis genes. DNA microarray analysis showed that the expression levels of several NtrC family genes, glnA, glnK, glnG, yhdX, yhdY, yhdZ, amtB, nac, argT and cbl were up-regulated and sucA, B, C, D genes were down-regulated. When (NH4)(2)SO4 was added at 40 g/l into the feeding solution, the final gamma-PGA concentration reached 3.7 g/l in the fed-batch culture of recombinant E. coli/pCOpgs. | - |
dc.description.sponsorship | This work was supported by the Basic Industrial Technology Development Project of the Korea Ministry of Commerce, Industry and Energy, National Research Laboratory Program of the Korea Ministry of Science and Technology, Center for Ultramicrochemical Process Systems, and by the BK21 project of Korea. | en |
dc.language | English | - |
dc.language.iso | en_US | en |
dc.publisher | SPRINGER | - |
dc.title | Optimal production of poly-gamma-glutamic acid by metabolically engineered Escherichia coli | - |
dc.type | Article | - |
dc.identifier.wosid | 000239085600004 | - |
dc.identifier.scopusid | 2-s2.0-33746059357 | - |
dc.type.rims | ART | - |
dc.citation.volume | 28 | - |
dc.citation.beginningpage | 1241 | - |
dc.citation.endingpage | 1246 | - |
dc.citation.publicationname | BIOTECHNOLOGY LETTERS | - |
dc.identifier.doi | 10.1007/s10529-006-9080-0 | - |
dc.embargo.liftdate | 9999-12-31 | - |
dc.embargo.terms | 9999-12-31 | - |
dc.contributor.localauthor | Lee, SangYup | - |
dc.contributor.nonIdAuthor | Jiang, H | - |
dc.contributor.nonIdAuthor | Shang, L | - |
dc.contributor.nonIdAuthor | Yoon, SH | - |
dc.contributor.nonIdAuthor | Yu, Z | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | Escherichia coli | - |
dc.subject.keywordAuthor | metabolic engineering | - |
dc.subject.keywordAuthor | nitrogen starvation | - |
dc.subject.keywordAuthor | poly-gamma-glutamic acid | - |
dc.subject.keywordAuthor | process optimization | - |
dc.subject.keywordPlus | BACILLUS-LICHENIFORMIS | - |
dc.subject.keywordPlus | CULTURE | - |
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